caa a22 4500 467900434 CHVBK 20180406152816.0 cr unu---uuuuu 170328e20060701xx s 000 0 eng 10.1007/s00299-005-0096-z doi (NATIONALLICENCE)springer-10.1007/s00299-005-0096-z Loss and recovery of protein productivity in genetically modified plant cell lines [Elektronische Daten] [Eddie James, James Lee] This paper addresses the problem of decreasing protein expression levels in genetically modified plant cells. A modeling approach was used to explain the loss of productivity over successive generations. Using this model, productivity losses were simulated for two cell lines. Although the lines were relatively stable, the protein production level could decrease by more than 80% after a large number of generations. Motivated by this problem, a dispersion method was developed to isolate productive cells from existing cell suspensions. Dilution of transgenic cells in a feeder layer of nonproducing cells at a ratio of 1:1000 facilitated the recovery of distinct, separate daughter colonies. Applying this method, it was possible to recover high producing cell lines. Springer-Verlag, 2005 Cloning nationallicence Dilution nationallicence Modeling nationallicence Plant cell nationallicence Protein production nationallicence Transgenic nationallicence IL : Interleukin · nationallicence GM-CSF : : Granulocyte-macrophage colony-stimulating factor · nationallicence NT : : Nicotiana tabacum nationallicence James Eddie School of Chemical Engineering and Bioengineering, Washington State University, 99164-2710, Pullman, WA, USA aut Lee James School of Chemical Engineering and Bioengineering, Washington State University, 99164-2710, Pullman, WA, USA aut Plant Cell Reports Springer-Verlag 25/7(2006-07-01), 723-727 0721-7714 25:7<723 2006 25 299 https://doi.org/10.1007/s00299-005-0096-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0096-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- James Eddie School of Chemical Engineering and Bioengineering, Washington State University, 99164-2710, Pullman, WA, USA aut NATIONALLICENCE 700 1- Lee James School of Chemical Engineering and Bioengineering, Washington State University, 99164-2710, Pullman, WA, USA aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/7(2006-07-01), 723-727 0721-7714 25:7<723 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer