caa a22 4500 467900450 CHVBK 20180406152816.0 cr unu---uuuuu 170328e20061201xx s 000 0 eng 10.1007/s00299-006-0198-2 doi (NATIONALLICENCE)springer-10.1007/s00299-006-0198-2 Regulation of isoflavone production in hydroponically grown Pueraria montana (kudzu) by cork pieces, XAD-4, and methyl jasmonate [Elektronische Daten] [Ara Kirakosyan, Peter Kaufman, Soo Chang, Sara Warber, Steven Bolling, Hrachik Vardapetyan] A mini-hydroponic growing system was employed for seedlings of kudzu vine (Pueraria montana) and contents of isoflavones (daidzein, genistein, daidzin, genistin, and puerarin) from shoot and root parts of seedlings were analyzed quantitatively. In addition, exogenous cork pieces, polymeric adsorbent, XAD-4, and universal elicitor, methyl jasmonate (MeJA), were used to regulate the production of these isoflavones. It was shown that cork pieces up-regulate the production of daidzein and genistein up to seven- and eight-fold greater than the levels obtained for control roots. In contrast, levels of glucosyl conjugates, daidzin and genistin, decrease up to five- and eight-fold, respectively. Cork treatment also induces the excretion of the root isoflavone constituents into the growth medium. Minimal levels of isoflavones are absorbed by the cork pieces. XAD-4 stimulates the production of glucosyl conjugates, daidzin and genistin, in root parts about 1.5-fold greater than that obtained in control roots. These are the highest amounts of daidzin and genistin that are observed (5.101 and 6.759mgg−1 dry weight, respectively). In contrast to these two adsorbents, MeJA increases the accumulation of isoflavones in shoot rather than in root parts of seedlings, about three- to four-fold over control levels, with the exception of genistein. These studies reveal new observations on the regulation of isoflavone production in hydroponically grown Pueraria montana plants by two adsorbents (cork pieces and XAD-4) and MeJA elicitor. Springer-Verlag, 2006 Kudzu vine ( Pueraria montana) nationallicence Secondary metabolite nationallicence Isoflavones nationallicence Cork pieces nationallicence XAD-4 nationallicence MeJA nationallicence Kirakosyan Ara Department of Cardiac Surgery, University of Michigan, 48109-0686, Ann Arbor, MI, USA aut Kaufman Peter Department of Cardiac Surgery, University of Michigan, 48109-0686, Ann Arbor, MI, USA aut Chang Soo University College, Yonsei University, 120-749, Seoul, Korea aut Warber Sara Michigan Integrative Medicine Program (UMIM), University of Michigan, 48104, Ann Arbor, MI, USA aut Bolling Steven Michigan Integrative Medicine Program (UMIM), University of Michigan, 48104, Ann Arbor, MI, USA aut Vardapetyan Hrachik Bio-Medical Faculty, Russian-Armenian (Slavonic) State University, 375051, Yerevan, Armenia aut Plant Cell Reports Springer-Verlag 25/12(2006-12-01), 1387-1391 0721-7714 25:12<1387 2006 25 299 https://doi.org/10.1007/s00299-006-0198-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0198-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kirakosyan Ara Department of Cardiac Surgery, University of Michigan, 48109-0686, Ann Arbor, MI, USA aut NATIONALLICENCE 700 1- Kaufman Peter Department of Cardiac Surgery, University of Michigan, 48109-0686, Ann Arbor, MI, USA aut NATIONALLICENCE 700 1- Chang Soo University College, Yonsei University, 120-749, Seoul, Korea aut NATIONALLICENCE 700 1- Warber Sara Michigan Integrative Medicine Program (UMIM), University of Michigan, 48104, Ann Arbor, MI, USA aut NATIONALLICENCE 700 1- Bolling Steven Michigan Integrative Medicine Program (UMIM), University of Michigan, 48104, Ann Arbor, MI, USA aut NATIONALLICENCE 700 1- Vardapetyan Hrachik Bio-Medical Faculty, Russian-Armenian (Slavonic) State University, 375051, Yerevan, Armenia aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/12(2006-12-01), 1387-1391 0721-7714 25:12<1387 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer