caa a22 4500 467900469 CHVBK 20180406152816.0 cr unu---uuuuu 170328e20061201xx s 000 0 eng 10.1007/s00299-006-0200-z doi (NATIONALLICENCE)springer-10.1007/s00299-006-0200-z Gynogenic plant regeneration from unpollinated flower explants of Eragrostis tef (Zuccagni) Trotter [Elektronische Daten] [Likyelesh Gugsa, Ashok Sarial, Horst Lörz, Jochen Kumlehn] Tef [Eragrostis tef (Zucc.) Trotter] is the most important cereal in Ethiopia. In its wild relative E. mexicana, regeneration of six green plants resulted from culture of 121 non-pollinated immature pistils. In the allotetraploid crop species tef, however, only callus and root formation was obtained by this method. By contrast, immature spikelets and panicle segments of E. tef proved amenable to gynogenic plant regeneration. Upon step-wise optimization of the protocol, efficient plant formation was achieved in all three cultivars tested. In cv. DZ-01-196, culture of 1305 immature spikelets resulted in formation of 159 green plants. Flow cytometric analysis revealed (di)haploid, triploid, tetraploid and octoploid regenerants, from which the vast majority was tetraploid. Tef-breeding programs will likely benefit substantially from efficient generation of true-breeding plants. Springer-Verlag, 2006 Embryogenesis nationallicence Gynogenesis nationallicence Haploid nationallicence Ovary nationallicence Pistil nationallicence Gugsa Likyelesh Leibniz Institute of Plant Genetics and Crop Plant Research, Corrensstrasse 3, 06466, Gatersleben, Germany aut Sarial Ashok Department of Biology, Addis Ababa University, P.O. Box 1176, Addis Ababa, Ethiopia aut Lörz Horst Department of Developmental Biology and Biotechnology, Biocenter Klein Flottbek, University of Hamburg, Ohnhorststrasse 18, 22609, Hamburg, Germany aut Kumlehn Jochen Leibniz Institute of Plant Genetics and Crop Plant Research, Corrensstrasse 3, 06466, Gatersleben, Germany aut Plant Cell Reports Springer-Verlag 25/12(2006-12-01), 1287-1293 0721-7714 25:12<1287 2006 25 299 https://doi.org/10.1007/s00299-006-0200-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0200-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gugsa Likyelesh Leibniz Institute of Plant Genetics and Crop Plant Research, Corrensstrasse 3, 06466, Gatersleben, Germany aut NATIONALLICENCE 700 1- Sarial Ashok Department of Biology, Addis Ababa University, P.O. Box 1176, Addis Ababa, Ethiopia aut NATIONALLICENCE 700 1- Lörz Horst Department of Developmental Biology and Biotechnology, Biocenter Klein Flottbek, University of Hamburg, Ohnhorststrasse 18, 22609, Hamburg, Germany aut NATIONALLICENCE 700 1- Kumlehn Jochen Leibniz Institute of Plant Genetics and Crop Plant Research, Corrensstrasse 3, 06466, Gatersleben, Germany aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/12(2006-12-01), 1287-1293 0721-7714 25:12<1287 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer