caa a22 4500 467900493 CHVBK 20180406152816.0 cr unu---uuuuu 170328e20061201xx s 000 0 eng 10.1007/s00299-006-0182-x doi (NATIONALLICENCE)springer-10.1007/s00299-006-0182-x In vitro plant regeneration from immature cotyledon explants of macadamia ( Macadamia tetraphylla L. Johnson) [Elektronische Daten] [Richard Mulwa, Prem Bhalla] The macadamia tree, an Australian native, is highly valued for its nuts. Macadamia improvement programs so far have relied on conventional breeding and selection. The production of improved cultivars required to meet future demands could be accelerated by the application of modern biotechnological techniques, but this requires an efficient and reproducible regeneration system that has not yet been established for macadamia. We report here shoot regeneration from immature cotyledon explants of macadamia. Adventitious buds were induced on the cotyledon explants from fruits collected at 140 and 190 days after full bloom (DAFB) on MS medium supplemented with either 10 or 15μM TDZ. The addition of 2% coconut milk (CM) to 10μM TDZ containing media resulted in enhanced adventitious bud induction from 190 DAFB explants. Further shoot development from the induced buds was depressed in media containing TDZ + CM; the addition of 0.001μM IAA to this combination doubled shoot development, from 1.9-3.9 shoots per explant. The transfer of bud clumps to media supplemented with 8.8μM BA alone or in combination with either 0.14μM GA3 or 0.001μM IAA significantly increased shoot production from the previously induced explants by 1.5-2 times of that observed in TDZ + CM medium. Histological examinations revealed that shoot regeneration was primarily by organogenesis originating from cells on or just below the cut surfaces of explants. Springer-Verlag, 2006 Macadamia nationallicence Somatic embryogenesis nationallicence Organogenesis nationallicence Histology nationallicence Mulwa Richard Plant Molecular Biology and Biotechnology Laboratory, Faculty of Land and Food Resources, Australian Research Council Centre of Excellence for Integrative Legume Research, The University of Melbourne, 3010, Parkville, Victoria, Australia aut Bhalla Prem Plant Molecular Biology and Biotechnology Laboratory, Faculty of Land and Food Resources, Australian Research Council Centre of Excellence for Integrative Legume Research, The University of Melbourne, 3010, Parkville, Victoria, Australia aut Plant Cell Reports Springer-Verlag 25/12(2006-12-01), 1281-1286 0721-7714 25:12<1281 2006 25 299 https://doi.org/10.1007/s00299-006-0182-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0182-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mulwa Richard Plant Molecular Biology and Biotechnology Laboratory, Faculty of Land and Food Resources, Australian Research Council Centre of Excellence for Integrative Legume Research, The University of Melbourne, 3010, Parkville, Victoria, Australia aut NATIONALLICENCE 700 1- Bhalla Prem Plant Molecular Biology and Biotechnology Laboratory, Faculty of Land and Food Resources, Australian Research Council Centre of Excellence for Integrative Legume Research, The University of Melbourne, 3010, Parkville, Victoria, Australia aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/12(2006-12-01), 1281-1286 0721-7714 25:12<1281 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer