caa a22 4500 467900507 CHVBK 20180406152816.0 cr unu---uuuuu 170328e20061201xx s 000 0 eng 10.1007/s00299-006-0155-0 doi (NATIONALLICENCE)springer-10.1007/s00299-006-0155-0 Genetic transformation of Centaurium erythraea Rafn by Agrobacterium rhizogenes and the production of secoiridoids [Elektronische Daten] [Ewelina Piatczak, Aleksandra Krolicka, Halina Wysokinska] Hairy roots of Centaurium erythraea were obtained by infection with Agrobacterium rhizogenes strain LBA 9402. They spontaneously regenerated adventitious shoots in Woody Plant liquid medium without growth regulators. The shoots were grown continuously in Murashige and Skoog (MS) liquid or agar solidified media supplemented with 0.1mgl−1 indole-3-acetic acid and 1.0mgl−1 6-benzylaminopurine. These shoots produced roots 4 weeks after transfer into agar-solidified MS medium without phytohormones. Regenerated plants grown and flowered under greenhouse conditions. The transgenic value of the regenerated plants was confirmed by the polymerase chain reaction amplification. Transformation by Agrobacterium rhizogenes alters plant morphology and production of secoiridoid glucosides. The level of secoiridoids was also modified by development stage of transformed plants. The total content of the compounds (expressed as the sum of gentiopicroside, sweroside and swertiamarin) in 10-week old pRi-transformed regenerants was 280mgg−1 dry weight and was 8-times the content in the sample of commercially available C. erythraea herb. Springer-Verlag, 2006 Agrobacterium rhizogenes nationallicence Centaurium erythraea Rafn nationallicence Ri-transformed plants nationallicence Secoiridoid glucosides nationallicence Piatczak Ewelina Department of Biology and Pharmaceutical Botany, Medical University of Lodz, Lodz, Poland aut Krolicka Aleksandra Faculty of Biotechnology, Department of Biotechnology, University of Gdansk and Medical University of Gdansk, Gdansk, Poland aut Wysokinska Halina Department of Biology and Pharmaceutical Botany, Medical University of Lodz, Lodz, Poland aut Plant Cell Reports Springer-Verlag 25/12(2006-12-01), 1308-1315 0721-7714 25:12<1308 2006 25 299 https://doi.org/10.1007/s00299-006-0155-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0155-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Piatczak Ewelina Department of Biology and Pharmaceutical Botany, Medical University of Lodz, Lodz, Poland aut NATIONALLICENCE 700 1- Krolicka Aleksandra Faculty of Biotechnology, Department of Biotechnology, University of Gdansk and Medical University of Gdansk, Gdansk, Poland aut NATIONALLICENCE 700 1- Wysokinska Halina Department of Biology and Pharmaceutical Botany, Medical University of Lodz, Lodz, Poland aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/12(2006-12-01), 1308-1315 0721-7714 25:12<1308 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer