caa a22 4500 467900515 CHVBK 20180406152816.0 cr unu---uuuuu 170328e20061201xx s 000 0 eng 10.1007/s00299-006-0166-x doi (NATIONALLICENCE)springer-10.1007/s00299-006-0166-x Establishment of a simple and efficient system for somatic embryo induction via ovule culture in Arabidopsis thaliana [Elektronische Daten] [Jun Wei, Xin-Ran Li, Meng-Xiang Sun] We established a simple and effective system to induce somatic embryos in Arabidopsis via ovule culture. Agar-solidified B5 basic medium supplemented with 10μ M 2,4-dichlorophenoxyacetic acid was used for callus induction. Ovules at all developmental stages were tested, and among these, ovules older than 48h after anthesis could be successfully induced to form embryogenic calli at high frequencies (42-82%). Structural and molecular probe analyses confirmed that the embryogenic calli were derived from embryos in the ovules. These calli were then easily induced to generate somatic embryos at frequencies of 63-95%. Subculture of the somatic embryos onto 1/2 strength MS medium resulted in their direct conversion into plants. The regenerants appeared morphologically normal and were fertile. This method provides a useful alternative tool to create sufficient numbers of somatic embryos for the study of biochemical and molecular mechanisms of embryogenesis, especially to recover early defective embryos in some mutations for cell-biological analyses. Springer-Verlag, 2006 Embryogenic callus nationallicence Ovule nationallicence Somatic embryo nationallicence CIM : Callus-induction medium nationallicence EC : Embryogenic calli nationallicence HAA : Hours after anthesis nationallicence SE : Somatic embryo nationallicence 2,4-D : 2,4-dichlorophenoxyacetic acid nationallicence Wei Jun Key Laboratory of MOE for Plant Development Biology, College of Life Science, Wuhan University, 430072, Wuhan, P.R. China aut Li Xin-Ran Key Laboratory of MOE for Plant Development Biology, College of Life Science, Wuhan University, 430072, Wuhan, P.R. China aut Sun Meng-Xiang Key Laboratory of MOE for Plant Development Biology, College of Life Science, Wuhan University, 430072, Wuhan, P.R. China aut Plant Cell Reports Springer-Verlag 25/12(2006-12-01), 1275-1280 0721-7714 25:12<1275 2006 25 299 https://doi.org/10.1007/s00299-006-0166-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0166-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wei Jun Key Laboratory of MOE for Plant Development Biology, College of Life Science, Wuhan University, 430072, Wuhan, P.R. China aut NATIONALLICENCE 700 1- Li Xin-Ran Key Laboratory of MOE for Plant Development Biology, College of Life Science, Wuhan University, 430072, Wuhan, P.R. China aut NATIONALLICENCE 700 1- Sun Meng-Xiang Key Laboratory of MOE for Plant Development Biology, College of Life Science, Wuhan University, 430072, Wuhan, P.R. China aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/12(2006-12-01), 1275-1280 0721-7714 25:12<1275 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer