caa a22 4500 467900523 CHVBK 20180406152816.0 cr unu---uuuuu 170328e20061201xx s 000 0 eng 10.1007/s00299-006-0210-x doi (NATIONALLICENCE)springer-10.1007/s00299-006-0210-x A red fluorescent protein, DsRed2, as a visual reporter for transient expression and stable transformation in soybean [Elektronische Daten] [Keito Nishizawa, Yoichi Kita, Masahiko Kitayama, Masao Ishimoto] Fluorescent proteins such as green fluorescent protein (GFP) from Aequorea victoria are often used as markers for transient expression and stable transformation in plants, given that their detection does not require a substrate and they can be monitored in a nondestructive manner. We have now evaluated the red fluorescent protein DsRed2 (a mutant form of DsRed from Discosoma sp.) for its suitability as a visual marker in combination with antibiotic selection for genetic transformation of soybean [Glycine max (L.) Merrill]. Transient and stable expression of DsRed2 in somatic embryos was readily detected by fluorescence microscopy, allowing easy confirmation of gene introduction. We obtained several fertile transgenic lines, including homozygous lines, that grew and produced seeds in an apparently normal manner. The red fluorescence of DsRed2 was detected by fluorescence microscopy without background fluorescence in both leaves and seeds of the transgenic plants. Furthermore, in contrast to seeds expressing GFP, those expressing DsRed2 were readily identifiable even under white light by the color conferred by the transgene product. The protein composition of seeds was not affected by the introduction of DsRed2, with the exception of the accumulation of DsRed2 itself, which was detectable as an additional band on electrophoresis. These results indicate that DsRed2 is a suitable reporter (even more suitable than GFP) for genetic transformation of soybean. Springer-Verlag, 2006 Bombardment nationallicence DsRed2 nationallicence Fluorescent protein nationallicence Nondestructive nationallicence Transgenic soybean nationallicence Nishizawa Keito National Agricultural Research Center for Hokkaido Region, 1 Hitsujigaoka, Toyohira, Sapporo, 062-8555, Hokkaido, Japan aut Kita Yoichi Ehime Women's College, 421 Ibuki, Uwajima, 798-0025, Ehime, Japan aut Kitayama Masahiko Ehime Women's College, 421 Ibuki, Uwajima, 798-0025, Ehime, Japan aut Ishimoto Masao National Agricultural Research Center for Hokkaido Region, 1 Hitsujigaoka, Toyohira, Sapporo, 062-8555, Hokkaido, Japan aut Plant Cell Reports Springer-Verlag 25/12(2006-12-01), 1355-1361 0721-7714 25:12<1355 2006 25 299 https://doi.org/10.1007/s00299-006-0210-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0210-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nishizawa Keito National Agricultural Research Center for Hokkaido Region, 1 Hitsujigaoka, Toyohira, Sapporo, 062-8555, Hokkaido, Japan aut NATIONALLICENCE 700 1- Kita Yoichi Ehime Women's College, 421 Ibuki, Uwajima, 798-0025, Ehime, Japan aut NATIONALLICENCE 700 1- Kitayama Masahiko Ehime Women's College, 421 Ibuki, Uwajima, 798-0025, Ehime, Japan aut NATIONALLICENCE 700 1- Ishimoto Masao National Agricultural Research Center for Hokkaido Region, 1 Hitsujigaoka, Toyohira, Sapporo, 062-8555, Hokkaido, Japan aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/12(2006-12-01), 1355-1361 0721-7714 25:12<1355 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer