caa a22 4500 467900531 CHVBK 20180406152816.0 cr unu---uuuuu 170328e20061201xx s 000 0 eng 10.1007/s00299-006-0188-4 doi (NATIONALLICENCE)springer-10.1007/s00299-006-0188-4 Transformation of barley ( Hordeum vulgare L.) by Agrobacterium tumefaciens infection of in vitro cultured ovules [Elektronische Daten] [Inger Holme, Henrik Brinch-Pedersen, Mette Lange, Preben Holm] We report on a novel transformation procedure for barley by Agrobacterium infection of in vitro cultured ovules. Ovules of the cultivar Golden Promise were isolated a few hours after pollination and infected with the Agrobacterium tumefaciens strain AGL0 carrying the binary vector pVec8-GFP. The vector harboured a hygromycin resistance gene and the green fluorescence protein (GFP) gene. GFP-expressing embryos were isolated from the ovules, regenerated to plants and investigated by Southern blot analysis. Transformation frequencies amounted to 3.1% with hygromycin selection and 0.8% without selection. Mendelian inheritance and stable expression of the GFP gene was confirmed in 18 independent lines over two generations. We conclude that the described technique allows for the rapid and direct generation of high quality transgenic plants. Springer-Verlag, 2006 Agrobacterium tumefaciens nationallicence Barley ovule culture nationallicence GFP-expression nationallicence Transformation nationallicence Zygote nationallicence Holme Inger Department of Genetics and Biotechnology, Danish Institute of Agricultural Sciences, Research Centre Flakkebjerg, 4200, Slagelse, Denmark aut Brinch-Pedersen Henrik Department of Genetics and Biotechnology, Danish Institute of Agricultural Sciences, Research Centre Flakkebjerg, 4200, Slagelse, Denmark aut Lange Mette Department of Genetics and Biotechnology, Danish Institute of Agricultural Sciences, Research Centre Flakkebjerg, 4200, Slagelse, Denmark aut Holm Preben Department of Genetics and Biotechnology, Danish Institute of Agricultural Sciences, Research Centre Flakkebjerg, 4200, Slagelse, Denmark aut Plant Cell Reports Springer-Verlag 25/12(2006-12-01), 1325-1335 0721-7714 25:12<1325 2006 25 299 https://doi.org/10.1007/s00299-006-0188-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0188-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Holme Inger Department of Genetics and Biotechnology, Danish Institute of Agricultural Sciences, Research Centre Flakkebjerg, 4200, Slagelse, Denmark aut NATIONALLICENCE 700 1- Brinch-Pedersen Henrik Department of Genetics and Biotechnology, Danish Institute of Agricultural Sciences, Research Centre Flakkebjerg, 4200, Slagelse, Denmark aut NATIONALLICENCE 700 1- Lange Mette Department of Genetics and Biotechnology, Danish Institute of Agricultural Sciences, Research Centre Flakkebjerg, 4200, Slagelse, Denmark aut NATIONALLICENCE 700 1- Holm Preben Department of Genetics and Biotechnology, Danish Institute of Agricultural Sciences, Research Centre Flakkebjerg, 4200, Slagelse, Denmark aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/12(2006-12-01), 1325-1335 0721-7714 25:12<1325 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer