caa a22 4500 467900574 CHVBK 20180406152816.0 cr unu---uuuuu 170328e20061201xx s 000 0 eng 10.1007/s00299-006-0206-6 doi (NATIONALLICENCE)springer-10.1007/s00299-006-0206-6 Bisexual sterility conferred by the differential expression of Barnase and Barstar: a simple and efficient method of transgene containment [Elektronische Daten] [Kappei Kobayashi, Ikuko Munemura, Kokichi Hinata, Saburo Yamamura] To establish a simple and an efficient system to minimize the environmental risk of genetically modified plants, we tested the applicability of the barnase/barstar system in conferring bisexual sterility; that is, in preventing plants setting seeds by self-fertilization and out-crossing. Transgenic tobacco plants were generated to express barnase, a cell death inducing ribonuclease, under the control of the gamete-specific AtDMC1 promoter, and barstar, a specific inhibitor of barnase, under the control of the ACT2 promoter, which is constitutively active in almost all tissues except gametes. In contrast to control plants harboring the barstar expression unit only, which set seeds normally with self-pollination, all transformants harboring both barnase and barstar were bisexually sterile. They produced aberrant anthers containing no detectable pollen and failed to set seeds even after pollination with wild-type tobacco pollen. Springer-Verlag, 2006 Environmental risk nationallicence Barnase nationallicence GMO nationallicence Sterility nationallicence Tobacco nationallicence Kobayashi Kappei Iwate Biotechnology Research Center, 22-174-4 Narita, Kitakami, 024-0003, Iwate, Japan aut Munemura Ikuko Iwate Biotechnology Research Center, 22-174-4 Narita, Kitakami, 024-0003, Iwate, Japan aut Hinata Kokichi Iwate Biotechnology Research Center, 22-174-4 Narita, Kitakami, 024-0003, Iwate, Japan aut Yamamura Saburo Iwate Biotechnology Research Center, 22-174-4 Narita, Kitakami, 024-0003, Iwate, Japan aut Plant Cell Reports Springer-Verlag 25/12(2006-12-01), 1347-1354 0721-7714 25:12<1347 2006 25 299 https://doi.org/10.1007/s00299-006-0206-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0206-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kobayashi Kappei Iwate Biotechnology Research Center, 22-174-4 Narita, Kitakami, 024-0003, Iwate, Japan aut NATIONALLICENCE 700 1- Munemura Ikuko Iwate Biotechnology Research Center, 22-174-4 Narita, Kitakami, 024-0003, Iwate, Japan aut NATIONALLICENCE 700 1- Hinata Kokichi Iwate Biotechnology Research Center, 22-174-4 Narita, Kitakami, 024-0003, Iwate, Japan aut NATIONALLICENCE 700 1- Yamamura Saburo Iwate Biotechnology Research Center, 22-174-4 Narita, Kitakami, 024-0003, Iwate, Japan aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/12(2006-12-01), 1347-1354 0721-7714 25:12<1347 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence SWISSBIB 467900477 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer