caa a22 4500 467900582 CHVBK 20180406152816.0 cr unu---uuuuu 170328e20061201xx s 000 0 eng 10.1007/s00299-006-0173-y doi (NATIONALLICENCE)springer-10.1007/s00299-006-0173-y Expression of active hBMP2 in transgenic tobacco plants [Elektronische Daten] [Guangli Suo, Bing Chen, Jingyu Zhang, Yuan Gao, Xia Wang, Zhengquan He, Jianwu Dai] Bone morphogenetic protein 2 (BMP2) is important for bone tissue repair. The goal of this research is to construct a high level human BMP2 (hBMP2) expression system using transgenic tobacco plants as a bioreactor. Cauliflower mosaic virus (CaMV) 35S promoter, alfalfa mosaic virus (AMV) enhancer, tobacco mosaic virus (TMV) enhancer, matrix attachment regions (MARs) sequence, and "Kozak” sequence were used to construct recombinant expression vectors and the high-expression vectors were screened out through GUS-fusions assay. The promoter is the most important factor; double-CaMV 35S promoter is more effective than single promoter. The AMV or TMV enhancer is able to promote the foreign protein expression. After four-step purification, the activated hBMP2 (0.02% total soluble protein) was obtained. Our results suggested that the transgenic tobacco has great potential to be used as a bioreactor to produce hBMP2. Springer-Verlag, 2006 Bone morphogenetic protein-2 nationallicence GUS fusion protein nationallicence Protein expression nationallicence Transgenic tobacco plants nationallicence Suo Guangli Center of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100080, Beijing, China aut Chen Bing Center of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100080, Beijing, China aut Zhang Jingyu Center of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100080, Beijing, China aut Gao Yuan Center of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100080, Beijing, China aut Wang Xia Center of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100080, Beijing, China aut He Zhengquan Biotechnology Research Center, China Three Gorges University, 443002, Yichang, China aut Dai Jianwu Center of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100080, Beijing, China aut Plant Cell Reports Springer-Verlag 25/12(2006-12-01), 1316-1324 0721-7714 25:12<1316 2006 25 299 https://doi.org/10.1007/s00299-006-0173-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0173-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Suo Guangli Center of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100080, Beijing, China aut NATIONALLICENCE 700 1- Chen Bing Center of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100080, Beijing, China aut NATIONALLICENCE 700 1- Zhang Jingyu Center of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100080, Beijing, China aut NATIONALLICENCE 700 1- Gao Yuan Center of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100080, Beijing, China aut NATIONALLICENCE 700 1- Wang Xia Center of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100080, Beijing, China aut NATIONALLICENCE 700 1- He Zhengquan Biotechnology Research Center, China Three Gorges University, 443002, Yichang, China aut NATIONALLICENCE 700 1- Dai Jianwu Center of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100080, Beijing, China aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/12(2006-12-01), 1316-1324 0721-7714 25:12<1316 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer