caa a22 4500 467900590 CHVBK 20180406152816.0 cr unu---uuuuu 170328e20061201xx s 000 0 eng 10.1007/s00299-006-0192-8 doi (NATIONALLICENCE)springer-10.1007/s00299-006-0192-8 Sequence stability of the T-DNA - plant junctions in tissue culture in Arabidopsis transgenic lines [Elektronische Daten] [Nina Papazova, Pieter Windels, Ann Depicker, Isabel Taverniers, Isabel Roldan-Ruiz, Anne Milcamps, Erik Van Bockstaele, Guy Van Den Eede, Marc De Loose] The stability of the inserted transgenes and particularly the junction regions of transgenic events is a concern of food labeling, traceability and post release monitoring, as these regions are used for development of event-specific DNA-based detection methods. During the standard agricultural breeding practices, the transgenic lines can be exposed to completely different conditions than those in the laboratory environment. Some of these conditions have the potential to affect the stability of the transgenic locus and the surrounding DNA. As tissue culture is recognized as a stressful and mutagenic factor, we have analyzed the effect of this process on the stability of the junction regions at nucleotide level in five Arabidopsis thaliana transgenic lines in comparison with the respective integration loci in ColO and C24 ecotypes. No indication of any kind of alteration at nucleotide level of the junctions was found. The relevance of the stability of the plant-T-DNA junction regions for application of the DNA-based methods in commercial transgenic plants is discussed. Springer-Verlag, 2006 Arabidopsis thaliana nationallicence GMO nationallicence Plant-T-DNA junctions nationallicence Sequencing nationallicence SNPs nationallicence Tissue culture nationallicence Papazova Nina Unit Technology and Food, Institute for Agricultural and Fisheries Research, Burg. Van Gansberghelaan 115, 9820, Merelbeke, Belgium aut Windels Pieter Unit Technology and Food, Institute for Agricultural and Fisheries Research, Burg. Van Gansberghelaan 115, 9820, Merelbeke, Belgium aut Depicker Ann VIB, Ghent University, Technologie Park 927, 9052, Zwijnaarde, Belgium aut Taverniers Isabel Unit Technology and Food, Institute for Agricultural and Fisheries Research, Burg. Van Gansberghelaan 115, 9820, Merelbeke, Belgium aut Roldan-Ruiz Isabel Unit Plant, Institute for Agricultural and Fisheries Research, Caritasstraat 21, 9090, Melle, Belgium aut Milcamps Anne European Commission - Joint Research Center, Institute of Health and Consumer Protection, 21020, Ispra, VA, Italy aut Van Bockstaele Erik Unit Plant, Institute for Agricultural and Fisheries Research, Caritasstraat 21, 9090, Melle, Belgium aut Van Den Eede Guy European Commission - Joint Research Center, Institute of Health and Consumer Protection, 21020, Ispra, VA, Italy aut De Loose Marc Unit Technology and Food, Institute for Agricultural and Fisheries Research, Burg. Van Gansberghelaan 115, 9820, Merelbeke, Belgium aut Plant Cell Reports Springer-Verlag 25/12(2006-12-01), 1362-1368 0721-7714 25:12<1362 2006 25 299 https://doi.org/10.1007/s00299-006-0192-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0192-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Papazova Nina Unit Technology and Food, Institute for Agricultural and Fisheries Research, Burg. Van Gansberghelaan 115, 9820, Merelbeke, Belgium aut NATIONALLICENCE 700 1- Windels Pieter Unit Technology and Food, Institute for Agricultural and Fisheries Research, Burg. Van Gansberghelaan 115, 9820, Merelbeke, Belgium aut NATIONALLICENCE 700 1- Depicker Ann VIB, Ghent University, Technologie Park 927, 9052, Zwijnaarde, Belgium aut NATIONALLICENCE 700 1- Taverniers Isabel Unit Technology and Food, Institute for Agricultural and Fisheries Research, Burg. Van Gansberghelaan 115, 9820, Merelbeke, Belgium aut NATIONALLICENCE 700 1- Roldan-Ruiz Isabel Unit Plant, Institute for Agricultural and Fisheries Research, Caritasstraat 21, 9090, Melle, Belgium aut NATIONALLICENCE 700 1- Milcamps Anne European Commission - Joint Research Center, Institute of Health and Consumer Protection, 21020, Ispra, VA, Italy aut NATIONALLICENCE 700 1- Van Bockstaele Erik Unit Plant, Institute for Agricultural and Fisheries Research, Caritasstraat 21, 9090, Melle, Belgium aut NATIONALLICENCE 700 1- Van Den Eede Guy European Commission - Joint Research Center, Institute of Health and Consumer Protection, 21020, Ispra, VA, Italy aut NATIONALLICENCE 700 1- De Loose Marc Unit Technology and Food, Institute for Agricultural and Fisheries Research, Burg. Van Gansberghelaan 115, 9820, Merelbeke, Belgium aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/12(2006-12-01), 1362-1368 0721-7714 25:12<1362 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer