caa a22 4500 467900612 CHVBK 20180406152817.0 cr unu---uuuuu 170328e20061201xx s 000 0 eng 10.1007/s00299-006-0205-7 doi (NATIONALLICENCE)springer-10.1007/s00299-006-0205-7 Efficient embryogenesis and regeneration in freshly isolated and cultured wheat ( Triticum aestivum L.) microspores without stress pretreatment [Elektronische Daten] [Mehran Shariatpanahi, Kristina Belogradova, Leila Hessamvaziri, Erwin Heberle-Bors, Alisher Touraev] The major advantage of doubled haploids in plant breeding is the immediate achievement of complete homozygosity. Desired genotypes are thus fixed in one generation, reducing time and cost for cultivar or inbred development. Among the different technologies to produce doubled haploids, microspore embryogenesis is by far the most common. It usually requires reprogramming of microspores by stress such as cold, heat, and starvation, followed by embryo development under stress-free conditions. We report here the development of a simple and efficient isolated microspore culture system for producing doubled haploid wheat plants in a wide spectrum of genotypes, in which embryogenic microspores and embryos are formed without any apparent stress treatment. Microspores were isolated from fresh spikes in a nutrient-free medium by stirring and cultured in medium A2 in the dark at 25°C. Once embryogenic microspores were formed, ovaries and phytohormones were added directly to the cultures without changing the medium. The cultures were incubated in the dark at 25-27°C until the formation of embryos and then the embryos were transferred to regeneration medium. The regeneration frequency and percentage of green plants increased significantly using this protocol compared to the shed microspore culture method. Springer-Verlag, 2006 Triticum aestivum nationallicence Microspores nationallicence Embryogenesis nationallicence Doubled haploids nationallicence Shariatpanahi Mehran Max F. Perutz Laboratories, University Departments at the Vienna Biocenter, Department of Plant Molecular Biology, Vienna University, A-1030, Dr. Bohrgasse 9, Vienna, Austria aut Belogradova Kristina Max F. Perutz Laboratories, University Departments at the Vienna Biocenter, Department of Plant Molecular Biology, Vienna University, A-1030, Dr. Bohrgasse 9, Vienna, Austria aut Hessamvaziri Leila Max F. Perutz Laboratories, University Departments at the Vienna Biocenter, Department of Plant Molecular Biology, Vienna University, A-1030, Dr. Bohrgasse 9, Vienna, Austria aut Heberle-Bors Erwin Max F. Perutz Laboratories, University Departments at the Vienna Biocenter, Department of Plant Molecular Biology, Vienna University, A-1030, Dr. Bohrgasse 9, Vienna, Austria aut Touraev Alisher Max F. Perutz Laboratories, University Departments at the Vienna Biocenter, Department of Plant Molecular Biology, Vienna University, A-1030, Dr. Bohrgasse 9, Vienna, Austria aut Plant Cell Reports Springer-Verlag 25/12(2006-12-01), 1294-1299 0721-7714 25:12<1294 2006 25 299 https://doi.org/10.1007/s00299-006-0205-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0205-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shariatpanahi Mehran Max F. Perutz Laboratories, University Departments at the Vienna Biocenter, Department of Plant Molecular Biology, Vienna University, A-1030, Dr. Bohrgasse 9, Vienna, Austria aut NATIONALLICENCE 700 1- Belogradova Kristina Max F. Perutz Laboratories, University Departments at the Vienna Biocenter, Department of Plant Molecular Biology, Vienna University, A-1030, Dr. Bohrgasse 9, Vienna, Austria aut NATIONALLICENCE 700 1- Hessamvaziri Leila Max F. Perutz Laboratories, University Departments at the Vienna Biocenter, Department of Plant Molecular Biology, Vienna University, A-1030, Dr. Bohrgasse 9, Vienna, Austria aut NATIONALLICENCE 700 1- Heberle-Bors Erwin Max F. Perutz Laboratories, University Departments at the Vienna Biocenter, Department of Plant Molecular Biology, Vienna University, A-1030, Dr. Bohrgasse 9, Vienna, Austria aut NATIONALLICENCE 700 1- Touraev Alisher Max F. Perutz Laboratories, University Departments at the Vienna Biocenter, Department of Plant Molecular Biology, Vienna University, A-1030, Dr. Bohrgasse 9, Vienna, Austria aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/12(2006-12-01), 1294-1299 0721-7714 25:12<1294 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer