caa a22 4500 467900620 CHVBK 20180406152817.0 cr unu---uuuuu 170328e20060301xx s 000 0 eng 10.1007/s00299-005-0043-z doi (NATIONALLICENCE)springer-10.1007/s00299-005-0043-z High efficiency plant production of North American ginseng via somatic embryogenesis from cotyledon explants [Elektronische Daten] [Sijun Zhou, Daniel Brown] An efficient in vitro protocol for plant production of North American ginseng has been established. The pretreatment of cotyledon explants with 1.0M sucrose at 4°C resulted in an improvement of embryo quality and, combined with a higher sucrose content (7%) in induction medium, improved the embryogenesis frequency from 40% to 75% and the number of embryos per explant from 10 to 21. The frequency of secondary embryogenesis from somatic embryo-derived tissues cultured on MS medium with 1.0 mgl−12, 4-D and 1.0mgl−1 NAA is up to 90%. Somatic embryos can further develop to maturity on SH medium supplemented with 1% activated charcoal and half of them can germinate. About 85% of the germinated embryos will convert into plants with well-developed taproot systems on ½ SH medium with 0.5% activated charcoal. The growth chamber and field establishment rates were 95.6 and 93.7%, respectively. The plants transplanted to growth chambers and field plots appear normal. Springer-Verlag, 2005 Embryo quality nationallicence Micropropagation nationallicence Panax quinquefolius nationallicence Plant regeneration nationallicence AC : Activated charcoal nationallicence B5 : Gamborg etal. (1968) nationallicence 2, 4-D : 2, 4-Dichlorophenoxyacetic acid nationallicence BA : 6-Benzyl-aminopurine nationallicence GA3 : Gibberellic acid nationallicence MS : Murashige and Skoog (1962) nationallicence NAA : α-Naphthaleneacetic acid nationallicence SH : Schenk and Hildebrandt (1972) nationallicence Zhou Sijun Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada, 1391 Sandford St., ON N5V 4T3, London, Canada aut Brown Daniel Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada, 1391 Sandford St., ON N5V 4T3, London, Canada aut Plant Cell Reports Springer-Verlag 25/3(2006-03-01), 166-173 0721-7714 25:3<166 2006 25 299 https://doi.org/10.1007/s00299-005-0043-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0043-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhou Sijun Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada, 1391 Sandford St., ON N5V 4T3, London, Canada aut NATIONALLICENCE 700 1- Brown Daniel Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada, 1391 Sandford St., ON N5V 4T3, London, Canada aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/3(2006-03-01), 166-173 0721-7714 25:3<166 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer