caa a22 4500 467900639 CHVBK 20180406152817.0 cr unu---uuuuu 170328e20060301xx s 000 0 eng 10.1007/s00299-005-0040-2 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0040-2 Green fluorescent protein as an efficient selection marker for Agrobacterium rhizogenes mediated carrot transformation [Elektronische Daten] [R. Baranski, E. Klocke, G. Schumann] Agrobacterium rhizogenes mediated transformation combined with a visual selection for green fluorescent protein (GFP) has been applied effectively in carrot (Daucus carota L.) transformation. Carrot root discs were inoculated with A4, A4T, LBA1334 and LBA9402 strains, all bearing gfp gene in pBIN-m-gfp5-ER. The results indicate that transformed adventitious roots can be visually selected solely based on GFP fluorescence with a very high accuracy. The method requires no selection agents like antibiotics or herbicides and enables a reduction of labour and time necessary for tissue culture. Moreover, individual transformants can be easily excised from the host tissue and cultured separately. All of the 12 used carrot cultivars produced transformed adventitious roots and the frequency of discs producing GFP expressing adventitious roots varied from 13 to 85%. The highest transformation rate was found for A4T and LBA1334 strains possessing chromosomal background of A. tumefaciens C58. The results encourage that visual selection of transformed, fluorescing adventitious roots can be highly effective and applied routinely for the production of carrot transgenic plants. Springer-Verlag, 2005 Agrobacterium rhizogenes nationallicence Daucus carota nationallicence GFP nationallicence Transformation efficiency nationallicence AS : Acetosyringone nationallicence GFP : Green fluorescent protein nationallicence NAA : α-Naphthaleneacetic acid nationallicence 2,4-D : 2,4-Dichlorophenoxyacetic acid nationallicence Baranski R. Department of Genetics, Plant Breeding and Seed Science, Faculty of Horticulture, Agricultural University of Krakow, Al. 29 Listopada 54, 31-425, Krakow, Poland aut Klocke E. Institute of Horticultural Crops, Federal Centre for Breeding Research on Cultivated Plants, Neuer Weg 22/23, 06484, Quedlinburg, Germany aut Schumann G. Institute of Horticultural Crops, Federal Centre for Breeding Research on Cultivated Plants, Neuer Weg 22/23, 06484, Quedlinburg, Germany aut Plant Cell Reports Springer-Verlag 25/3(2006-03-01), 190-197 0721-7714 25:3<190 2006 25 299 https://doi.org/10.1007/s00299-005-0040-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0040-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Baranski R. Department of Genetics, Plant Breeding and Seed Science, Faculty of Horticulture, Agricultural University of Krakow, Al. 29 Listopada 54, 31-425, Krakow, Poland aut NATIONALLICENCE 700 1- Klocke E. Institute of Horticultural Crops, Federal Centre for Breeding Research on Cultivated Plants, Neuer Weg 22/23, 06484, Quedlinburg, Germany aut NATIONALLICENCE 700 1- Schumann G. Institute of Horticultural Crops, Federal Centre for Breeding Research on Cultivated Plants, Neuer Weg 22/23, 06484, Quedlinburg, Germany aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/3(2006-03-01), 190-197 0721-7714 25:3<190 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer