caa a22 4500 467900647 CHVBK 20180406152817.0 cr unu---uuuuu 170328e20060301xx s 000 0 eng 10.1007/s00299-005-0048-7 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0048-7 Improved cotyledonary node method using an alternative explant derived from mature seed for efficient Agrobacterium -mediated soybean transformation [Elektronische Daten] [Margie Paz, Juan Martinez, Andrea Kalvig, Tina Fonger, Kan Wang] The utility of transformation for soybean improvement requires an efficient system for production of stable transgenic lines. We describe here an improved cotyledonary node method using an alternative explant for Agrobacterium tumefaciens-mediated soybean transformation. We use the term "half-seed” to refer to this alternative cotyledonary explant that is derived from mature seed of soybean following an overnight imbibition and to distinguish it from cotyledonary node derived from 5-7-day-old seedlings. Transformation efficiencies using half-seed explants ranged between 1.4 and 8.7% with an overall efficiency of 3.8% based on the number of transformed events that have been confirmed in the T 1 generation by phenotypic assay using the herbicide Liberty® (active ingredient glufosinate) and by Southern analysis. This efficiency is 1.5-fold higher than the cotyledonary node method used in our laboratory. Significantly, the half-seed system is simple and does not require deliberate wounding of explants, which is a critical and technically demanding step in the cotyledonary node method. Springer-Verlag, 2005 Agrobacterium tumefaciens nationallicence Cotyledonary node nationallicence Glufosinate nationallicence Half-seed nationallicence Soybean nationallicence Transformation nationallicence Paz Margie Department of Agronomy, Iowa State University, 50011-1010, Ames, IA, USA aut Martinez Juan Department of Agronomy, Iowa State University, 50011-1010, Ames, IA, USA aut Kalvig Andrea Department of Agronomy, Iowa State University, 50011-1010, Ames, IA, USA aut Fonger Tina Department of Agronomy, Iowa State University, 50011-1010, Ames, IA, USA aut Wang Kan Department of Agronomy, Iowa State University, 50011-1010, Ames, IA, USA aut Plant Cell Reports Springer-Verlag 25/3(2006-03-01), 206-213 0721-7714 25:3<206 2006 25 299 https://doi.org/10.1007/s00299-005-0048-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0048-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Paz Margie Department of Agronomy, Iowa State University, 50011-1010, Ames, IA, USA aut NATIONALLICENCE 700 1- Martinez Juan Department of Agronomy, Iowa State University, 50011-1010, Ames, IA, USA aut NATIONALLICENCE 700 1- Kalvig Andrea Department of Agronomy, Iowa State University, 50011-1010, Ames, IA, USA aut NATIONALLICENCE 700 1- Fonger Tina Department of Agronomy, Iowa State University, 50011-1010, Ames, IA, USA aut NATIONALLICENCE 700 1- Wang Kan Department of Agronomy, Iowa State University, 50011-1010, Ames, IA, USA aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/3(2006-03-01), 206-213 0721-7714 25:3<206 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence SWISSBIB 467900647 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer