caa a22 4500 46790068X CHVBK 20180406152817.0 cr unu---uuuuu 170328e20060301xx s 000 0 eng 10.1007/s00299-005-0044-y doi (NATIONALLICENCE)springer-10.1007/s00299-005-0044-y In vitro culture methods in sorghum with shoot tip as the explant material [Elektronische Daten] [N Sai, KBRS Visarada, Y. Lakshmi, E. Pashupatinath, S. Rao, N. Seetharama] Twenty-four diverse genotypes of sorghum were evaluated for response to callus induction and plant regeneration with two media viz., MS and NBKNB using shoot tips as the start material to identify a model genotype. None of the genotypes tested showed promising results. Therefore, alternative methods of in vitro pathways using shoot meristem isolated from shoot tips were explored. Shoot apical meristems were isolated and were induced to multiple shoots or multiple shoot buds pathway by manipulation of thidiazuron (TDZ), 6-benzyl adenine (BAP) and 2, 4-dichlorophenoxy acetic acid (2, 4-D). Choice of the pathway whether large-scale multiplication of shoots or production of target tissues for transformation can be exercised based on the needs and applications. A simple procedure, for large scale handling of shoot tips is described in detail. Electron microscopic studies revealed that meristems isolated from 7-day-old seedlings are superior because of possessing greater number of transformation competent cells. Springer-Verlag, 2005 Shoot meristems nationallicence Multiple shoot buds nationallicence Electron microscopy nationallicence Thidiazuron nationallicence Benzyladenine nationallicence Callus nationallicence Sai N. National Research Center for Sorghum (NRCS), Rajendranagar, 500030, Hyderabad, Andhra Pradesh, India aut Visarada KBRS National Research Center for Sorghum (NRCS), Rajendranagar, 500030, Hyderabad, Andhra Pradesh, India aut Lakshmi Y. National Research Center for Sorghum (NRCS), Rajendranagar, 500030, Hyderabad, Andhra Pradesh, India aut Pashupatinath E. National Research Center for Sorghum (NRCS), Rajendranagar, 500030, Hyderabad, Andhra Pradesh, India aut Rao S. National Research Center for Sorghum (NRCS), Rajendranagar, 500030, Hyderabad, Andhra Pradesh, India aut Seetharama N. National Research Center for Sorghum (NRCS), Rajendranagar, 500030, Hyderabad, Andhra Pradesh, India aut Plant Cell Reports Springer-Verlag 25/3(2006-03-01), 174-182 0721-7714 25:3<174 2006 25 299 https://doi.org/10.1007/s00299-005-0044-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0044-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sai N. National Research Center for Sorghum (NRCS), Rajendranagar, 500030, Hyderabad, Andhra Pradesh, India aut NATIONALLICENCE 700 1- Visarada KBRS National Research Center for Sorghum (NRCS), Rajendranagar, 500030, Hyderabad, Andhra Pradesh, India aut NATIONALLICENCE 700 1- Lakshmi Y. National Research Center for Sorghum (NRCS), Rajendranagar, 500030, Hyderabad, Andhra Pradesh, India aut NATIONALLICENCE 700 1- Pashupatinath E. National Research Center for Sorghum (NRCS), Rajendranagar, 500030, Hyderabad, Andhra Pradesh, India aut NATIONALLICENCE 700 1- Rao S. National Research Center for Sorghum (NRCS), Rajendranagar, 500030, Hyderabad, Andhra Pradesh, India aut NATIONALLICENCE 700 1- Seetharama N. National Research Center for Sorghum (NRCS), Rajendranagar, 500030, Hyderabad, Andhra Pradesh, India aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/3(2006-03-01), 174-182 0721-7714 25:3<174 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer