caa a22 4500 467900698 CHVBK 20180406152817.0 cr unu---uuuuu 170328e20060301xx s 000 0 eng 10.1007/s00299-005-0042-0 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0042-0 Transformation of a muskmelon ‘Galia' hybrid parental line ( Cucumis melo L. var. reticulatus Ser.) with an antisense ACC oxidase gene [Elektronische Daten] [Hector Nuñez-Palenius, Daniel Cantliffe, Don Huber, Joseph Ciardi, Harry Klee] ‘Galia' muskmelon (Cucumis melo L. var. reticulatus Ser.) has been recalcitrant to transformation by Agrobacterium tumefaciens. Transformation of the ‘Galia' male parental line, ‘Krymka', with an ACC oxidase (CMACO-1) gene in antisense orientation is described herein. Explants were transformed using A. tumefaciens strain ABI, which contained a vector pCmACO1-AS plasmid, bearing an antisense gene of CMACO-1 and the CP4 syn gene (glyphosate-tolerance). Both CMACO-1 and CP4 syn genes were assessed by a polymerase chain reaction method. Flow cytometry analysis was performed to determine plant ploidy level of primary transformants. Two completely diploid independent transgenic plants were obtained. Southern blot and segregation analysis in the T1 generation determined that each independent transgenic line had one single insertion of the transgene. These transgenic muskmelon male parental lines have potential for use in the production of ‘Galia' F1 hybrids with improved shelf life. Springer-Verlag, 2005 Ethylene nationallicence ‘Galia' nationallicence Organogenesis nationallicence Plant tissue culture nationallicence Agrobacterium nationallicence Glyphosate nationallicence Fruit ripening nationallicence ACC : 1-Aminocyclopropane-1-carboxylic acid nationallicence ACO-1 : ACC oxidase-1 nationallicence CMACO-1 : Cucumis melo ACO-1 nationallicence BA : 6-Benzylaminopurine nationallicence IAA : Indole-3-acetic acid nationallicence MS : Murashige and Skoog medium nationallicence Nuñez-Palenius Hector Horticultural Sciences Department, University of Florida, IFAS, 32611, Gainesville, FL, USA aut Cantliffe Daniel Horticultural Sciences Department, University of Florida, IFAS, 32611, Gainesville, FL, USA aut Huber Don Horticultural Sciences Department, University of Florida, IFAS, 32611, Gainesville, FL, USA aut Ciardi Joseph Horticultural Sciences Department, University of Florida, IFAS, 32611, Gainesville, FL, USA aut Klee Harry Horticultural Sciences Department, University of Florida, IFAS, 32611, Gainesville, FL, USA aut Plant Cell Reports Springer-Verlag 25/3(2006-03-01), 198-205 0721-7714 25:3<198 2006 25 299 https://doi.org/10.1007/s00299-005-0042-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0042-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nuñez-Palenius Hector Horticultural Sciences Department, University of Florida, IFAS, 32611, Gainesville, FL, USA aut NATIONALLICENCE 700 1- Cantliffe Daniel Horticultural Sciences Department, University of Florida, IFAS, 32611, Gainesville, FL, USA aut NATIONALLICENCE 700 1- Huber Don Horticultural Sciences Department, University of Florida, IFAS, 32611, Gainesville, FL, USA aut NATIONALLICENCE 700 1- Ciardi Joseph Horticultural Sciences Department, University of Florida, IFAS, 32611, Gainesville, FL, USA aut NATIONALLICENCE 700 1- Klee Harry Horticultural Sciences Department, University of Florida, IFAS, 32611, Gainesville, FL, USA aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/3(2006-03-01), 198-205 0721-7714 25:3<198 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer