caa a22 4500 46790071X CHVBK 20180406152817.0 cr unu---uuuuu 170328e20060301xx s 000 0 eng 10.1007/s00299-005-0056-7 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0056-7 Characterization of an anther- and tapetum-specific gene and its highly specific promoter isolated from tomato [Elektronische Daten] [S. Xu, G. Liu, R. Chen] A full-length genomic clone of 2,233bp long containing an anther- and tapetum-specific gene TomA108 was isolated and characterized from tomato. The gene was present in one copy per haploid genome. The isolated clone contained 5′ and 3′ untranslated regions of 810 and 170 nucleotides, respectively and a single intron with highly repetitive sequences. The cDNA encoded the protein with an apparent mass of 10.6kDa and a pI (isoelectric point) of 5.3. It was cysteine-rich and had an N-terminal hydrophobic domain with characteristics of a secretory signal. Amino acid sequence comparisons demonstrated that the protein was closely related to a family of cereal seed storage proteins and protease inhibitors. The fusion of β-glucuronidase to the TomA108 promoter demonstrated that the promoter was highly active from early-meiosis to free microspores production in tapetum of tobacco. This strong and highly specific promoter can be potentially used to generate male sterility for efficient production of plant hybrids. Springer-Verlag, 2005 Anther nationallicence Cysteine-rich nationallicence Promoter nationallicence Tapetum nationallicence GUS : β-Glucuronidase nationallicence EDTA : Ethylene diamine tetraacetie acid nationallicence MADS : MCM1, AGAMOUS, DEFICIENS, and SRF nationallicence CarG : (CC(A/T)6GG) nationallicence GA : Gibbrerllin nationallicence ABA : Abscisic acid nationallicence SA : Salicylic acid nationallicence SDS : Sodium dodecyl sulfate nationallicence IPTG : Isopropyl-1-thio-β-d-alactoside nationallicence PAGE : Polyacrylamide gel electrophoresis nationallicence Xu S. Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, China aut Liu G. Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, China aut Chen R. Department of Biochemistry, University of Saskatchewan, 107 Wiggins Road, SK S7N 5E5, Saskatoon, Canada aut Plant Cell Reports Springer-Verlag 25/3(2006-03-01), 231-240 0721-7714 25:3<231 2006 25 299 https://doi.org/10.1007/s00299-005-0056-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0056-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xu S. Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, China aut NATIONALLICENCE 700 1- Liu G. Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, China aut NATIONALLICENCE 700 1- Chen R. Department of Biochemistry, University of Saskatchewan, 107 Wiggins Road, SK S7N 5E5, Saskatoon, Canada aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/3(2006-03-01), 231-240 0721-7714 25:3<231 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer