caa a22 4500 467900736 CHVBK 20180406152817.0 cr unu---uuuuu 170328e20060301xx s 000 0 eng 10.1007/s00299-005-0063-8 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0063-8 Silencing an ACC oxidase gene affects the susceptible host response of Nicotiana benthamiana to infection by Colletotrichum orbiculare [Elektronische Daten] [X. Shan, P. Goodwin] An 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase, NbACO1, was cloned from cDNA of Nicotiana benthamiana leaves infected with the hemibiotrophic fungal pathogen, Colletotrichum orbiculare. Expression of NbACO1 increased approximately 3-fold by 24h after inoculation and then continued to increase reaching a maximum that was 6-folds greater than that in healthy plants by 96h after inoculation. A portion of NbACO1 was cloned into a PVX vector for virus-induced gene silencing, and the silencing resulted in a reduction in its expression to 7-9% of that found in the controls for fungal-infected leaf tissue. Silencing of NbACO1 also resulted in significant reductions in transcript levels of genes encoding an ethylene responsive transcription factor and two glutathione S-transferases, but not for a basic pathogenesis-related protein gene, indicating that at least some genes associated with ethylene signaling were affected by the silencing treatment. Inoculated NbACO1-silenced plants developed more lesions more quickly as a result of an accelerated switch from the symptomless, biotrophic phase to the symptomatic necrotrophic phase of infection compared to inoculated control plants. This indicates that manipulation of ACC oxidase can affect the length of the biotrophic phase of infection in this interaction. Springer-Verlag, 2005 1-Aminocyclopropane-1-carboxylic acid oxidase nationallicence Ethylene nationallicence Hemibiotrophy nationallicence cDNA : Complimentary DNA nationallicence RACE : Rapid amplification of cDNA ends nationallicence HR : Hypersensitive response nationallicence RT-PCR : Reverse transcriptase PCR nationallicence SYAS : Sodium chloride-yeast extract-sucrose agar nationallicence VIGS : Virus-induced gene silencing nationallicence Shan X. Department of Environmental Biology, University of Guelph, NIG 2W1, Guelph, Ontario, Canada aut Goodwin P. Department of Environmental Biology, University of Guelph, NIG 2W1, Guelph, Ontario, Canada aut Plant Cell Reports Springer-Verlag 25/3(2006-03-01), 241-247 0721-7714 25:3<241 2006 25 299 https://doi.org/10.1007/s00299-005-0063-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0063-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shan X. Department of Environmental Biology, University of Guelph, NIG 2W1, Guelph, Ontario, Canada aut NATIONALLICENCE 700 1- Goodwin P. Department of Environmental Biology, University of Guelph, NIG 2W1, Guelph, Ontario, Canada aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/3(2006-03-01), 241-247 0721-7714 25:3<241 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer