caa a22 4500 467900744 CHVBK 20180406152817.0 cr unu---uuuuu 170328e20061001xx s 000 0 eng 10.1007/s00299-006-0148-z doi (NATIONALLICENCE)springer-10.1007/s00299-006-0148-z Refining the application of direct embryogenesis in sugarcane: effect of the developmental phase of leaf disc explants and the timing of DNA transfer on transformation efficiency [Elektronische Daten] [S. Snyman, G. Meyer, J. Richards, N. Haricharan, S. Ramgareeb, B. Huckett] A rapid in vitro protocol using direct somatic embryogenesis and microprojectile bombardment was investigated to establish the developmental phases most suitable for efficient sugarcane transformation. Immature leaf roll disc explants with and without pre-emergent inflorescence tissue were compared. It was shown that for effective transformation to occur, explants should be cultured for several days to allow initiation of embryo development prior to bombardment. Leaf roll discs with pre-emergent inflorescences showed a higher degree of embryogenic competence than non-flowering explants, and transformation efficiency was higher when explants containing floral initials were bombarded. Despite the occurrence of high numbers of phenotypically negative plants, combining the use of inflorescent leaf roll discs with direct embryogenic regeneration has the potential to improve the speed and efficiency of transgenesis in sugarcane. Springer-Verlag, 2006 Direct somatic embryogenesis nationallicence Pre-emergent inflorescence nationallicence Sugarcane transformation nationallicence Snyman S. South African Sugarcane Research Institute, Private Bag X02, 4300, Mt. Edgecombe, South Africa aut Meyer G. South African Sugarcane Research Institute, Private Bag X02, 4300, Mt. Edgecombe, South Africa aut Richards J. South African Sugarcane Research Institute, Private Bag X02, 4300, Mt. Edgecombe, South Africa aut Haricharan N. South African Sugarcane Research Institute, Private Bag X02, 4300, Mt. Edgecombe, South Africa aut Ramgareeb S. South African Sugarcane Research Institute, Private Bag X02, 4300, Mt. Edgecombe, South Africa aut Huckett B. South African Sugarcane Research Institute, Private Bag X02, 4300, Mt. Edgecombe, South Africa aut Plant Cell Reports Springer-Verlag 25/10(2006-10-01), 1016-1023 0721-7714 25:10<1016 2006 25 299 https://doi.org/10.1007/s00299-006-0148-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0148-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Snyman S. South African Sugarcane Research Institute, Private Bag X02, 4300, Mt. Edgecombe, South Africa aut NATIONALLICENCE 700 1- Meyer G. South African Sugarcane Research Institute, Private Bag X02, 4300, Mt. Edgecombe, South Africa aut NATIONALLICENCE 700 1- Richards J. South African Sugarcane Research Institute, Private Bag X02, 4300, Mt. Edgecombe, South Africa aut NATIONALLICENCE 700 1- Haricharan N. South African Sugarcane Research Institute, Private Bag X02, 4300, Mt. Edgecombe, South Africa aut NATIONALLICENCE 700 1- Ramgareeb S. South African Sugarcane Research Institute, Private Bag X02, 4300, Mt. Edgecombe, South Africa aut NATIONALLICENCE 700 1- Huckett B. South African Sugarcane Research Institute, Private Bag X02, 4300, Mt. Edgecombe, South Africa aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/10(2006-10-01), 1016-1023 0721-7714 25:10<1016 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer