caa a22 4500 467900752 CHVBK 20180406152817.0 cr unu---uuuuu 170328e20061001xx s 000 0 eng 10.1007/s00299-006-0169-7 doi (NATIONALLICENCE)springer-10.1007/s00299-006-0169-7 Screening and expression analysis of Phytophthora infestans induced genes in potato leaves with horizontal resistance [Elektronische Daten] [Z. Tian, J. Liu, B. Wang, C. Xie] Horizontal resistance to late blight with quantitative and durable characteristics is a major objective for potato breeding programs. With the aim of investigating the molecular aspects of horizontal resistance, a cDNA microarray was used to identify Phytophthora infestans-induced genes from 100 expressed sequence tags (ESTs) selected from a subtractive cDNA library. Of the 100 cDNA clones represented on the array, 76 were differentially expressed in infected plants as compared with mock-inoculated control plants. Four groups of genes could be identified according to their expression patterns at three time points, 24, 48 and 72h postinoculation (hpi). Group A appeared to be strongly induced (>10-fold) at 72hpi. Group B demonstrated up-regulated expression patterns at all the three time points. The transcripts of group C peaked at 48hpi, while genes of group D were up-regulated at 24hpi and decreased slightly thereafter. Blast algorithm searches revealed that the largest set of up-regulated genes (about 35%) was assigned to the primary/secondary metabolism. Other genes with known or putative functions included disease defense or cell rescue (about 18%), transcription, signal transduction, cellular transporter/transport facilitation, development, protein synthesis/destination, as well as those playing roles in cellular organization. Furthermore, 15 genes encoding unknown function proteins were also identified. The results indicated that multiple defense mechanisms are involved in horizontal potato resistance to late blight and alteration in metabolic pathways is one of the most important defense responses. Springer-Verlag, 2006 Horizontal resistance nationallicence Late blight nationallicence Pathogen-induced gene nationallicence Phytophthora infestans nationallicence Solanum tuberosum L nationallicence Tian Z. Key Laboratory of Horticultural Plant Biology, Ministry of Education/National Center for Vegetable Improvement (Central China), Huazhong Agricultural University, 430070, Wuhan, Hubei, People's Republic of China aut Liu J. Key Laboratory of Horticultural Plant Biology, Ministry of Education/National Center for Vegetable Improvement (Central China), Huazhong Agricultural University, 430070, Wuhan, Hubei, People's Republic of China aut Wang B. Key Laboratory of Horticultural Plant Biology, Ministry of Education/National Center for Vegetable Improvement (Central China), Huazhong Agricultural University, 430070, Wuhan, Hubei, People's Republic of China aut Xie C. Key Laboratory of Horticultural Plant Biology, Ministry of Education/National Center for Vegetable Improvement (Central China), Huazhong Agricultural University, 430070, Wuhan, Hubei, People's Republic of China aut Plant Cell Reports Springer-Verlag 25/10(2006-10-01), 1094-1103 0721-7714 25:10<1094 2006 25 299 https://doi.org/10.1007/s00299-006-0169-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0169-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tian Z. Key Laboratory of Horticultural Plant Biology, Ministry of Education/National Center for Vegetable Improvement (Central China), Huazhong Agricultural University, 430070, Wuhan, Hubei, People's Republic of China aut NATIONALLICENCE 700 1- Liu J. Key Laboratory of Horticultural Plant Biology, Ministry of Education/National Center for Vegetable Improvement (Central China), Huazhong Agricultural University, 430070, Wuhan, Hubei, People's Republic of China aut NATIONALLICENCE 700 1- Wang B. Key Laboratory of Horticultural Plant Biology, Ministry of Education/National Center for Vegetable Improvement (Central China), Huazhong Agricultural University, 430070, Wuhan, Hubei, People's Republic of China aut NATIONALLICENCE 700 1- Xie C. Key Laboratory of Horticultural Plant Biology, Ministry of Education/National Center for Vegetable Improvement (Central China), Huazhong Agricultural University, 430070, Wuhan, Hubei, People's Republic of China aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/10(2006-10-01), 1094-1103 0721-7714 25:10<1094 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer