caa a22 4500 467900760 CHVBK 20180406152817.0 cr unu---uuuuu 170328e20061001xx s 000 0 eng 10.1007/s00299-006-0171-0 doi (NATIONALLICENCE)springer-10.1007/s00299-006-0171-0 GISH and AFLP analyses of novel Brassica napus lines derived from one hybrid between B. napus and Orychophragmus violaceus [Elektronische Daten] [Ni Ma, Zai-Yun Li, J. Cartagena, K. Fukui] New Brassica napus inbred lines with different petal colors and with canola quality and increased levels of oleic (∼70%, 10% higher than that of B. napus parent) and linoleic (28%) acids have been developed in the progenies of one B. napus cv. Oro × Orychophragmus violaceus F5 hybrid plant (2n=31). Their genetic constituents were analyzed by using the methods of genomic in situ hybridization (GISH) and amplified fragments length polymorphism (AFLP). No intact chromosomes of O. violaceus origin were detected by GISH in their somatic cells of ovaries and root tips (2n=38) and pollen mother cells (PMCs) with normal chromosome pairing (19 bivalents) and segregation (19:19), though signals of variable sizes and intensities were located mainly at terminal and centromeric parts of some mitotic chromosomes and meiotic bivalents at diakinesis or chromosomes in anaphase I groups and one large patch of chromatin was intensively labeled and separated spatially in some telophase I nuclei and metaphase II PMCs. AFLP analysis revealed that substantial genomic changes have occurred in these lines and O. violaceus-specific bands, deleted bands in ‘Oro' and novel bands for two parents were detected. The possible mechanisms for these results were discussed. Springer-Verlag, 2006 Amplified fragments length polymorphism (AFLP) nationallicence Brassica napus nationallicence Genetic introgression nationallicence Genomic in situ hybridization (GISH) nationallicence Orychophragmus violaceus nationallicence Ma Ni National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding Technology, National Center of Oil Crop Improvement (Wuhan), College of Plant Science and Technology, Huazhong Agricultural University, 430070, Wuhan, P. R. China aut Li Zai-Yun National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding Technology, National Center of Oil Crop Improvement (Wuhan), College of Plant Science and Technology, Huazhong Agricultural University, 430070, Wuhan, P. R. China aut Cartagena J. Department of Biotechnology, Graduate School of Engineering, Osaka University, Yamada-Oka 2-1, 565-0871, Suita, Japan aut Fukui K. Department of Biotechnology, Graduate School of Engineering, Osaka University, Yamada-Oka 2-1, 565-0871, Suita, Japan aut Plant Cell Reports Springer-Verlag 25/10(2006-10-01), 1089-1093 0721-7714 25:10<1089 2006 25 299 https://doi.org/10.1007/s00299-006-0171-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0171-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ma Ni National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding Technology, National Center of Oil Crop Improvement (Wuhan), College of Plant Science and Technology, Huazhong Agricultural University, 430070, Wuhan, P. R. China aut NATIONALLICENCE 700 1- Li Zai-Yun National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding Technology, National Center of Oil Crop Improvement (Wuhan), College of Plant Science and Technology, Huazhong Agricultural University, 430070, Wuhan, P. R. China aut NATIONALLICENCE 700 1- Cartagena J. Department of Biotechnology, Graduate School of Engineering, Osaka University, Yamada-Oka 2-1, 565-0871, Suita, Japan aut NATIONALLICENCE 700 1- Fukui K. Department of Biotechnology, Graduate School of Engineering, Osaka University, Yamada-Oka 2-1, 565-0871, Suita, Japan aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/10(2006-10-01), 1089-1093 0721-7714 25:10<1089 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer