caa a22 4500 467900779 CHVBK 20180406152817.0 cr unu---uuuuu 170328e20061001xx s 000 0 eng 10.1007/s00299-006-0145-2 doi (NATIONALLICENCE)springer-10.1007/s00299-006-0145-2 Improved Agrobacterium -mediated transformation of three maize inbred lines using MS salts [Elektronische Daten] [Bronwyn R. Frame, Jennifer M. McMurray, Tina M. Fonger, Marcy L. Main, Kyle W. Taylor, François J. Torney, Margie M. Paz, Kan Wang] Transformation technology as a research or breeding tool to improve maize is routinely used in most industrial and some specialized public laboratories. However, transformation of many inbred lines remains a challenging task, especially when using Agrobacterium tumefaciens as the delivery method. Here we report success in generating transgenic plants and progeny from three maize inbred lines using an Agrobacterium-mediated standard binary vector system to target maize immature embryos. Eleven maize inbred lines were pre-screened for transformation frequency using N6 salts. A subset of three maize inbred lines was then systematically evaluated for frequency of post-infection embryogenic callus induction and transformation on four media regimes: N6 or MS salts in each of two distinct media backgrounds. Transgenic plants recovered from inbred lines B104, B114, and Ky21 were analyzed for transgene integration, expression, and transmission. Average transformation frequencies of 6.4% (for B104), 2.8% (for B114), and 8% (for Ky21) were achieved using MS salts. Availability of Agrobacterium-mediated maize inbred line transformation will improve future opportunities for maize genetic and functional genomic studies. Springer-Verlag, 2006 Agrobacterium tumefaciens nationallicence Zea mays nationallicence Inbred maize nationallicence MS salts nationallicence N6 salts nationallicence Type I callus nationallicence Frame Bronwyn R. Department of Agronomy, Iowa State University, Ames, IA 50011-1010, USA aut McMurray Jennifer M. Department of Agronomy, Iowa State University, Ames, IA 50011-1010, USA aut Fonger Tina M. Department of Agronomy, Iowa State University, Ames, IA 50011-1010, USA aut Main Marcy L. Department of Agronomy, Iowa State University, Ames, IA 50011-1010, USA aut Taylor Kyle W. Department of Agronomy, Iowa State University, Ames, IA 50011-1010, USA aut Torney François J. Department of Agronomy, Iowa State University, Ames, IA 50011-1010, USA aut Paz Margie M. Department of Agronomy, Iowa State University, Ames, IA 50011-1010, USA aut Wang Kan Department of Agronomy, Iowa State University, Ames, IA 50011-1010, USA aut Plant Cell Reports Springer-Verlag 25/10(2006-10-01), 1024-1034 0721-7714 25:10<1024 2006 25 299 https://doi.org/10.1007/s00299-006-0145-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0145-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Frame Bronwyn R. Department of Agronomy, Iowa State University, Ames, IA 50011-1010, USA aut NATIONALLICENCE 700 1- McMurray Jennifer M. Department of Agronomy, Iowa State University, Ames, IA 50011-1010, USA aut NATIONALLICENCE 700 1- Fonger Tina M. Department of Agronomy, Iowa State University, Ames, IA 50011-1010, USA aut NATIONALLICENCE 700 1- Main Marcy L. Department of Agronomy, Iowa State University, Ames, IA 50011-1010, USA aut NATIONALLICENCE 700 1- Taylor Kyle W. Department of Agronomy, Iowa State University, Ames, IA 50011-1010, USA aut NATIONALLICENCE 700 1- Torney François J. Department of Agronomy, Iowa State University, Ames, IA 50011-1010, USA aut NATIONALLICENCE 700 1- Paz Margie M. Department of Agronomy, Iowa State University, Ames, IA 50011-1010, USA aut NATIONALLICENCE 700 1- Wang Kan Department of Agronomy, Iowa State University, Ames, IA 50011-1010, USA aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/10(2006-10-01), 1024-1034 0721-7714 25:10<1024 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer