caa a22 4500 467900795 CHVBK 20180406152817.0 cr unu---uuuuu 170328e20061001xx s 000 0 eng 10.1007/s00299-006-0164-z doi (NATIONALLICENCE)springer-10.1007/s00299-006-0164-z Spontaneous plant regeneration in transformed roots and calli from Tylophora indica [Elektronische Daten] changes in morphological phenotype and tylophorine accumulation associated with transformation by Agrobacterium rhizogenes [Kuntal Chaudhuri, Biswajit Ghosh, David Tepfer, Sumita Jha] We examined the effects of genetic transformation by Agrobacterium rhizogenes on the production of tylophorine, a phenanthroindolizidine alkaloid, in the Indian medicinal plant, Tylophora indica. Transformed roots induced by the bacterium grew in axenic culture and produced shoots or embryogenic calli in the absence of hormone treatments. However, hormonal treatment was required to regenerate shoots in root explants of wild type control plants. Transformed plants showed morphological features typically seen in transgenic plants produced by A. rhizogenes, which include, short internodes, small and wrinkled leaves, more branches and numerous plagiotropic roots. Plants regenerated from transformed roots showed increased biomass accumulation (350-510% in the roots and 200-320% in the whole plants) and augmented tylophorine content (20-60%) in the shoots, resulting in a 160-280% increase in tylophorine production in different clones grown in vitro. Springer-Verlag, 2006 Agrobacterium rhizogenes nationallicence Medicinal plants nationallicence Plant regeneration nationallicence Tylophora indica nationallicence Tylophorine nationallicence BA : 6-Benzyl adenine nationallicence BM : Basal medium nationallicence HPLC : High performance liquid chromatography nationallicence Ri : Root-inducing nationallicence T-DNA : Transferred DNA nationallicence TL-DNA : Left-handed T-DNA nationallicence WT : Wild type nationallicence Chaudhuri Kuntal Centre of Advanced Study in Cell and Chromosome Research, Department of Botany, University of Calcutta, 35 Ballygunge Circular Road, 700019, Calcutta, India aut Ghosh Biswajit Department of Botany, RKMVC College, Rahara, 24 Parganas (North), West Bengal, India aut Tepfer David Laboratoire de Biologie de la Rhizosphère (Phytopharmacie), Institut National de la Recherche Agronomique, 78026, Versailles cédex, France aut Jha Sumita Centre of Advanced Study in Cell and Chromosome Research, Department of Botany, University of Calcutta, 35 Ballygunge Circular Road, 700019, Calcutta, India aut Plant Cell Reports Springer-Verlag 25/10(2006-10-01), 1059-1066 0721-7714 25:10<1059 2006 25 299 https://doi.org/10.1007/s00299-006-0164-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0164-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chaudhuri Kuntal Centre of Advanced Study in Cell and Chromosome Research, Department of Botany, University of Calcutta, 35 Ballygunge Circular Road, 700019, Calcutta, India aut NATIONALLICENCE 700 1- Ghosh Biswajit Department of Botany, RKMVC College, Rahara, 24 Parganas (North), West Bengal, India aut NATIONALLICENCE 700 1- Tepfer David Laboratoire de Biologie de la Rhizosphère (Phytopharmacie), Institut National de la Recherche Agronomique, 78026, Versailles cédex, France aut NATIONALLICENCE 700 1- Jha Sumita Centre of Advanced Study in Cell and Chromosome Research, Department of Botany, University of Calcutta, 35 Ballygunge Circular Road, 700019, Calcutta, India aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/10(2006-10-01), 1059-1066 0721-7714 25:10<1059 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer