caa a22 4500 467900809 CHVBK 20180406152817.0 cr unu---uuuuu 170328e20061001xx s 000 0 eng 10.1007/s00299-006-0158-x doi (NATIONALLICENCE)springer-10.1007/s00299-006-0158-x Investigation of the endosperm-specific sucrose synthase promoter from rice using transient expression of reporter genes in guar seed tissue [Elektronische Daten] [Thomas Rasmussen, Iain Donaldson] We report the investigation of an endosperm-specific promoter from the rsus3 gene from rice (Oryza sativa). The promoter was characterized by deletion analysis and transient expression in guar (Cyamopsis tetragonoloba) seed-tissue. Transient expression was monitored by histochemical GUS assay, and quantitative dual reporter assays comprising firefly luciferase as a test reporter, and Renilla luciferase and GUS as reference reporters. These revealed high expression levels of the reporter genes directed by the rsus3 promoter in guar endosperm. Specificity for this tissue in seeds was apparent from a virtual absence of reporter activity in guar cotyledons. Removal of a putative intron region only slightly raised the expression level, whereas duplication of the minimal promoter region, in a tandem-repeat rsus3 promoter construct, retained endosperm specificity in guar, and displayed three times the reporter activity observed with the single copy construct. Springer-Verlag, 2006 Dual reporter nationallicence Endosperm nationallicence Guar nationallicence Luciferase nationallicence Rsus3 promoter nationallicence Transient expression nationallicence Rasmussen Thomas Danisco Innovation, Langebrogade 1, 1001, Copenhagen K, Denmark aut Donaldson Iain Danisco Innovation, Langebrogade 1, 1001, Copenhagen K, Denmark aut Plant Cell Reports Springer-Verlag 25/10(2006-10-01), 1035-1042 0721-7714 25:10<1035 2006 25 299 https://doi.org/10.1007/s00299-006-0158-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0158-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rasmussen Thomas Danisco Innovation, Langebrogade 1, 1001, Copenhagen K, Denmark aut NATIONALLICENCE 700 1- Donaldson Iain Danisco Innovation, Langebrogade 1, 1001, Copenhagen K, Denmark aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/10(2006-10-01), 1035-1042 0721-7714 25:10<1035 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer