caa a22 4500 467900825 CHVBK 20180406152817.0 cr unu---uuuuu 170328e20061001xx s 000 0 eng 10.1007/s00299-006-0154-1 doi (NATIONALLICENCE)springer-10.1007/s00299-006-0154-1 Developmental and hormonal regulation of direct shoot organogenesis and somatic embryogenesis in sugarcane ( Saccharum spp. interspecific hybrids) leaf culture [Elektronische Daten] [Prakash Lakshmanan, R. Geijskes, Lifang Wang, Adrian Elliott, Christopher Grof, Nils Berding, Grant Smith] Rapid and efficient in vitro regeneration methods that minimise somaclonal variation are critical for the genetic transformation and mass propagation of commercial varieties. Using a transverse thin cell layer culture system, we have identified some of the developmental and physiological constraints that limit high-frequency regeneration in sugarcane leaf tissue. Tissue polarity and consequently the orientation of the explant in culture, size and developmental phase of explant, and auxin concentration play a significant role in determining the organogenic potential of leaf tissue in culture. Both adventitious shoot production and somatic embryogenesis occurred on the proximal cut surface of the explant, and a regeneration gradient, decreasing gradually from the basal to the distal end, exists in the leaf roll. Importantly, auxin, when added to the culture medium, reduced this spatial developmental constraint, as well as the effect of genotype on plant regeneration. Transverse sections (1-2mm thick) obtained from young leaf spindle rolls and orienting explants with its distal end facing the medium (directly in contact with medium) are critical for maximum regeneration. Shoot regeneration was observed as early as 3 weeks on MS medium supplemented with α-naphthalenencetic acid (NAA) and 6-benzyladenine, while somatic embryogenesis or both adventitious shoot organogenesis and somatic embryogenesis occurred on medium with NAA and chlorophenoxyacetic acid. Twenty shoots or more could be generated from a single transverse section explant. These shoots regenerated roots and successfully established after transplanted to pots. Large numbers of plantlets can be regenerated directly and rapidly using this system. SmartSett®, the registered name for this process and the plants produced, will have significant practical applications for the mass propagation of new cultivars and in genetic modification programs. The SmartSett® system has already been used commercially to produce substantial numbers of plants of orange rust-resistant and new cultivars in Australia. Springer-Verlag, 2006 Direct regeneration nationallicence Saccharum nationallicence SmartSett® nationallicence Somaclonal variation nationallicence Somatic embryogenesis nationallicence Sugarcane nationallicence Thin cell layer culture nationallicence Lakshmanan Prakash David North Plant Research Centre, BSES Limited, 50 Meiers Road, Indooroopilly, 4068, Brisbane, Queensland, Australia aut Geijskes R. David North Plant Research Centre, BSES Limited, 50 Meiers Road, Indooroopilly, 4068, Brisbane, Queensland, Australia aut Wang Lifang David North Plant Research Centre, BSES Limited, 50 Meiers Road, Indooroopilly, 4068, Brisbane, Queensland, Australia aut Elliott Adrian CSIRO-Plant Industry, Queensland Bioscience Precinct, 306 Carmody Road, 4067, St Lucia, Queensland, Australia aut Grof Christopher CSIRO-Plant Industry, Queensland Bioscience Precinct, 306 Carmody Road, 4067, St Lucia, Queensland, Australia aut Berding Nils Meringa Sugar Experiment Station, BSES Limited, Bruce Highway, 4865, Gordonvale, Queensland, Australia aut Smith Grant David North Plant Research Centre, BSES Limited, 50 Meiers Road, Indooroopilly, 4068, Brisbane, Queensland, Australia aut Plant Cell Reports Springer-Verlag 25/10(2006-10-01), 1007-1015 0721-7714 25:10<1007 2006 25 299 https://doi.org/10.1007/s00299-006-0154-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0154-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lakshmanan Prakash David North Plant Research Centre, BSES Limited, 50 Meiers Road, Indooroopilly, 4068, Brisbane, Queensland, Australia aut NATIONALLICENCE 700 1- Geijskes R. David North Plant Research Centre, BSES Limited, 50 Meiers Road, Indooroopilly, 4068, Brisbane, Queensland, Australia aut NATIONALLICENCE 700 1- Wang Lifang David North Plant Research Centre, BSES Limited, 50 Meiers Road, Indooroopilly, 4068, Brisbane, Queensland, Australia aut NATIONALLICENCE 700 1- Elliott Adrian CSIRO-Plant Industry, Queensland Bioscience Precinct, 306 Carmody Road, 4067, St Lucia, Queensland, Australia aut NATIONALLICENCE 700 1- Grof Christopher CSIRO-Plant Industry, Queensland Bioscience Precinct, 306 Carmody Road, 4067, St Lucia, Queensland, Australia aut NATIONALLICENCE 700 1- Berding Nils Meringa Sugar Experiment Station, BSES Limited, Bruce Highway, 4865, Gordonvale, Queensland, Australia aut NATIONALLICENCE 700 1- Smith Grant David North Plant Research Centre, BSES Limited, 50 Meiers Road, Indooroopilly, 4068, Brisbane, Queensland, Australia aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/10(2006-10-01), 1007-1015 0721-7714 25:10<1007 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer