caa a22 4500 467900833 CHVBK 20180406152817.0 cr unu---uuuuu 170328e20061001xx s 000 0 eng 10.1007/s00299-006-0185-7 doi (NATIONALLICENCE)springer-10.1007/s00299-006-0185-7 Expression of a polyubiquitin promoter isolated from Gladiolus [Elektronische Daten] [Young Hee Joung, Kathryn Kamo] A polyubiquitin promoter (GUBQ1) including its 5′UTR and intron was isolated from the floral monocot Gladiolus because high levels of expression could not be obtained using publicly available promoters isolated from either cereals or dicots. Sequencing of the promoter revealed highly conserved 5′ and 3′ intron splicing sites for the 1.234kb intron. The coding sequence of the first two ubiquitin genes showed the highest homology (87 and 86%, respectively) to the ubiquitin genes of Nicotiana tabacum and Oryza sativa RUBQ2. Transient expression following gene gun bombardment showed that relative levels of GUS activity with the GUBQ1 promoter were comparable to the CaMV 35S promoter in gladiolus, tobacco, rose, rice, and the floral monocot freesia. The highest levels of GUS expression with GUBQ1 were attained with Gladiolus. The full-length GUBQ1 promoter including 5′UTR and intron were necessary for maximum GUS expression in Gladiolus. The relative GUS activity for the promoter only was 9%, and the activity for the promoter with 5′UTR and 399bp of the full-length 1.234kb intron was 41%. Arabidopsis plants transformed with uidA under GUBQ1 showed moderate GUS expression throughout young leaves and in the vasculature of older leaves. The highest levels of transient GUS expression in Gladiolus have been achieved using the GUBQ1 promoter. This promoter should be useful for genetic engineering of disease resistance in Gladiolus, rose, and freesia, where high levels of gene expression are important. Springer-Verlag, 2006 Intron nationallicence Calla lily nationallicence Freesia nationallicence Cannas nationallicence Lily nationallicence Rice nationallicence Tobacco nationallicence Joung Young Hee School of Biological Science and Technology, Chonnam National University, 500-757, Gwangju, Korea aut Kamo Kathryn U.S. Department of Agriculture, U.S. National Arboretum, Floral and Nursery Plants Research Unit, 20705-2350, Beltsville, MD, USA aut Plant Cell Reports Springer-Verlag 25/10(2006-10-01), 1081-1088 0721-7714 25:10<1081 2006 25 299 https://doi.org/10.1007/s00299-006-0185-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0185-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Joung Young Hee School of Biological Science and Technology, Chonnam National University, 500-757, Gwangju, Korea aut NATIONALLICENCE 700 1- Kamo Kathryn U.S. Department of Agriculture, U.S. National Arboretum, Floral and Nursery Plants Research Unit, 20705-2350, Beltsville, MD, USA aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/10(2006-10-01), 1081-1088 0721-7714 25:10<1081 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer