caa a22 4500 46790085X CHVBK 20180406152817.0 cr unu---uuuuu 170328e20061001xx s 000 0 eng 10.1007/s00299-006-0161-2 doi (NATIONALLICENCE)springer-10.1007/s00299-006-0161-2 The shikonin derivatives and pyrrolizidine alkaloids in hairy root cultures of Lithospermum canescens (Michx.) Lehm [Elektronische Daten] [A. Pietrosiuk, K. Sykłowska-Baranek, H. Wiedenfeld, R. Wolinowska, M. Furmanowa, E. Jaroszyk] Hairy root cultures of Lithospermum canescens were established using three strains of Agrobacterium rhizogenes: ATCC 15834, LBA 9402 and NCIB 8196. Eight lines resulting from infection with A. rhizogenes ATCC 15834 demonstrated sufficient biomass increase and were submitted to further investigations. The contents of acetylshikonin (ACS) and isobutyrylshikonin (IBS) in transformed hairy roots made up ca. 10% of those observed in natural roots of L. canescens (24.35 and 14.48mgg−1 DW, respectively). One line, Lc1-D, produced the largest amounts of ACS (2.72mgg−1 DW) and IBS (0.307mgg−1 DW). Traces of pyrrolizidine alkaloids (PA), canescine and canescenine, were found in all lines of transformed hairy roots. Springer-Verlag, 2006 Agrobacterium rhizogenes nationallicence Hairy roots nationallicence Lithospermum canescens nationallicence Pyrrolizidine alkaloids nationallicence Shikonin derivatives nationallicence ACS : acetylshikonin nationallicence DW : dry weight nationallicence IBS : isobutyrylshikonin nationallicence PA : pyrrolizidine alkaloids nationallicence Pietrosiuk A. Department of Biology and Pharmaceutical Botany, Medical University of Warsaw, ul. Banacha 1, 02-097, Warsaw, Poland aut Sykłowska-Baranek K. Department of Biology and Pharmaceutical Botany, Medical University of Warsaw, ul. Banacha 1, 02-097, Warsaw, Poland aut Wiedenfeld H. Pharmazeutisches Institut der Universität, An der Immenburg 4, D-53121, Bonn, Germany aut Wolinowska R. Department of Microbiology, Medical University of Warsaw, ul. Oczki 3, 02-007, Warsaw, Poland aut Furmanowa M. Department of Biology and Pharmaceutical Botany, Medical University of Warsaw, ul. Banacha 1, 02-097, Warsaw, Poland aut Jaroszyk E. Department of Biology and Pharmaceutical Botany, Medical University of Warsaw, ul. Banacha 1, 02-097, Warsaw, Poland aut Plant Cell Reports Springer-Verlag 25/10(2006-10-01), 1052-1058 0721-7714 25:10<1052 2006 25 299 https://doi.org/10.1007/s00299-006-0161-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0161-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pietrosiuk A. Department of Biology and Pharmaceutical Botany, Medical University of Warsaw, ul. Banacha 1, 02-097, Warsaw, Poland aut NATIONALLICENCE 700 1- Sykłowska-Baranek K. Department of Biology and Pharmaceutical Botany, Medical University of Warsaw, ul. Banacha 1, 02-097, Warsaw, Poland aut NATIONALLICENCE 700 1- Wiedenfeld H. Pharmazeutisches Institut der Universität, An der Immenburg 4, D-53121, Bonn, Germany aut NATIONALLICENCE 700 1- Wolinowska R. Department of Microbiology, Medical University of Warsaw, ul. Oczki 3, 02-007, Warsaw, Poland aut NATIONALLICENCE 700 1- Furmanowa M. Department of Biology and Pharmaceutical Botany, Medical University of Warsaw, ul. Banacha 1, 02-097, Warsaw, Poland aut NATIONALLICENCE 700 1- Jaroszyk E. Department of Biology and Pharmaceutical Botany, Medical University of Warsaw, ul. Banacha 1, 02-097, Warsaw, Poland aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/10(2006-10-01), 1052-1058 0721-7714 25:10<1052 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer