caa a22 4500 467900876 CHVBK 20180406152817.0 cr unu---uuuuu 170328e20061001xx s 000 0 eng 10.1007/s00299-006-0149-y doi (NATIONALLICENCE)springer-10.1007/s00299-006-0149-y Gibberellins and antheridiogen on sex in Blechnum spicant L [Elektronische Daten] [V. Menéndez, M. Revilla, P. Bernard, V. Gotor, H. Fernández] The role of gibberellins (GAs) in determining sex in the gametophyte of the fern Blechnum spicant L. was studied through (a) the effect of exogenous GA4+7 and GA3 (b) quantitation of the endogenous levels of GA1, GA3, GA4, GA7, GA9, and GA20 in male and female gametophytes, and (c) the effect of flurprimidol, a GAs biosynthesis inhibitor of the steps of oxidation of ent-kaureno to ent-kaurenoic acid. Our results show that GA4+7 had a slight effect of inducing either male or female sexual organs, antheridia and archegonia, respectively. The endogenous GAs content was not significantly different between sexes, but the GA4, GA7, and GA20 levels were raised above those of the other GAs in both sexes. Neither antheridiogen biosynthesis nor antheridia formation was inhibited by flurprimidol. Gametophytes regenerated from homogenized mature gametophytes (HG) show a different physiological behavior than spore-derived gametophytes. In the first case, gametophytes are males and synthesize antheridiogen before they attain maturity, in contrast to what occurs in spore-derived gametophytes which are females and synthesize antheridiogen when mature. Springer-Verlag, 2006 Antheridiogen nationallicence Blechnum spicant nationallicence Fern nationallicence Gibberellins nationallicence Sexual development nationallicence ABA : abscisic acid nationallicence AC : active charcoal nationallicence DW : dry weight nationallicence GAs : gibberellins nationallicence HG : homogenized mature gametophytes nationallicence HPLC : high performance liquid chromatography nationallicence MS : Murashige and Skoog mineral medium (1962) nationallicence WMS : MS medium without the cellular contents released during homogenization nationallicence m / z : ratio of mass to charge nationallicence Menéndez V. Laboratory of Plant Physiology, Department of B.O.S., c) Catedrático R. Uría s/n, 33071, Oviedo, Spain aut Revilla M. Laboratory of Plant Physiology, Department of B.O.S., c) Catedrático R. Uría s/n, 33071, Oviedo, Spain aut Bernard P. Department of Química Orgánica e Inorgánica, c) Julián Clavería s/n, 33071, Oviedo, Spain aut Gotor V. Laboratory of Plant Physiology, Department of B.O.S., c) Catedrático R. Uría s/n, 33071, Oviedo, Spain aut Fernández H. Laboratory of Plant Physiology, Department of B.O.S., c) Catedrático R. Uría s/n, 33071, Oviedo, Spain aut Plant Cell Reports Springer-Verlag 25/10(2006-10-01), 1104-1110 0721-7714 25:10<1104 2006 25 299 https://doi.org/10.1007/s00299-006-0149-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0149-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Menéndez V. Laboratory of Plant Physiology, Department of B.O.S., c) Catedrático R. Uría s/n, 33071, Oviedo, Spain aut NATIONALLICENCE 700 1- Revilla M. Laboratory of Plant Physiology, Department of B.O.S., c) Catedrático R. Uría s/n, 33071, Oviedo, Spain aut NATIONALLICENCE 700 1- Bernard P. Department of Química Orgánica e Inorgánica, c) Julián Clavería s/n, 33071, Oviedo, Spain aut NATIONALLICENCE 700 1- Gotor V. Laboratory of Plant Physiology, Department of B.O.S., c) Catedrático R. Uría s/n, 33071, Oviedo, Spain aut NATIONALLICENCE 700 1- Fernández H. Laboratory of Plant Physiology, Department of B.O.S., c) Catedrático R. Uría s/n, 33071, Oviedo, Spain aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/10(2006-10-01), 1104-1110 0721-7714 25:10<1104 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer