caa a22 4500 467900892 CHVBK 20180406152817.0 cr unu---uuuuu 170328e20060401xx s 000 0 eng 10.1007/s00299-005-0064-7 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0064-7 Microspore embryogenesis and the development of a double haploidy protocol for cow cockle ( Saponaria vaccaria ) [Elektronische Daten] [Zoë Kernan, A. Ferrie] Factors affecting microspore embryogenesis of cow cockle (Saponaria vaccaria) were evaluated including donor plant growing conditions, genotype, bud size, density, medium composition, and culture conditions. Of the two donor plant (day/night) temperature regimes evaluated (10/5°C and 20/15°C), plants grown at 20/15°C were the most embryogenic. An embryogenic frequency of greater than 350 embryos/100 buds was observed in the most embryogenic genotype, cv. ‘White Beauty'. Buds from 3-9mm in length were evaluated for their embryogenic potential; buds that were 4-7.9mm produced the most embryos/100 buds. Of all the media compositions evaluated, NLN medium with 15% sucrose resulted in the most embryos. Cow cockle microspores required an initial period of 32°C for 3 days for production of microspore-derived embryos (MDEs). Springer-Verlag, 2005 Cow cockle nationallicence Haploids nationallicence Microspore culture nationallicence Microspore embryogenesis nationallicence Saponaria vaccaria nationallicence MDEs : Derived microspore embryos nationallicence DH : Double haploid nationallicence Kernan Zoë National Research Council/Plant Biotechnology Institute, 110 Gymnasium Place, S7N 0W9, Saskatoon, SK, Canada aut Ferrie A. National Research Council/Plant Biotechnology Institute, 110 Gymnasium Place, S7N 0W9, Saskatoon, SK, Canada aut Plant Cell Reports Springer-Verlag 25/4(2006-04-01), 274-280 0721-7714 25:4<274 2006 25 299 https://doi.org/10.1007/s00299-005-0064-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0064-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kernan Zoë National Research Council/Plant Biotechnology Institute, 110 Gymnasium Place, S7N 0W9, Saskatoon, SK, Canada aut NATIONALLICENCE 700 1- Ferrie A. National Research Council/Plant Biotechnology Institute, 110 Gymnasium Place, S7N 0W9, Saskatoon, SK, Canada aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/4(2006-04-01), 274-280 0721-7714 25:4<274 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer