caa a22 4500 467900922 CHVBK 20180406152817.0 cr unu---uuuuu 170328e20060401xx s 000 0 eng 10.1007/s00299-005-0047-8 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0047-8 Production of doubled haploids in durum wheat ( Triticum turgidum L.) through isolated microspore culture [Elektronische Daten] [L. Cistué, M. Soriano, A. Castillo, M. Vallés, J. Sanz, B. Echávarri] The objective of this work was to produce doubled haploid plants from durum wheat through the induction of androgenesis. A microspore culture technique was developed and used to produce fertile doubled haploid plants of agronomic interest. Five cultivars, one selected line, plus a collection of 20 F1 crosses between different genotypes of high breeding value were used. Studies on several factors such as pre-treatments and media components were carried out in order to develop a protocol to regenerate green haploid plantlets. Anthers were pre-treated in 0.7M mannitol. Microspores, from anther maceration, were plated on a C17 induction culture medium with ovary co-culture. The optimum regeneration medium J25-8 was used. From 35 microspore isolations, 407 green plantlets were obtained. With this technique mature embryos were obtained. Green plants were regenerated from all genotypes used and approximately 67% of them were spontaneously doubled haploids. Some haploids and a very few polyploids plants were obtained. From the 407 plants, 275 were completely fertile and gave enough seeds to be assayed in the field. This protocol could be used complementary to or instead of the intergeneric crossing with maize as an economically feasible method to obtain doubled haploids from most durum wheat genotypes. Springer-Verlag, 2005 Doubled haploid nationallicence Microspore culture nationallicence Durum wheat nationallicence Triticum turgidum nationallicence DH : Doubled haploid nationallicence 2,4-D : 2,4-dic-hlorophenoxyacetic acid nationallicence BAP : 6-Benzylaminopurine nationallicence KIN : 6-Furfurylaminopurine nationallicence NAA : Naphthalene acetic acid nationallicence PAA : Phenylacetic acid nationallicence Cistué L. Departamento de Genética y Producción Vegetal, Estación Experimental de Aula Dei, Consejo Superior de Investigaciones Científicas, Avda. Montañana 1005, 50059, Zaragoza, Spain aut Soriano M. Departamento de Genética y Producción Vegetal, Estación Experimental de Aula Dei, Consejo Superior de Investigaciones Científicas, Avda. Montañana 1005, 50059, Zaragoza, Spain aut Castillo A. Departamento de Genética y Producción Vegetal, Estación Experimental de Aula Dei, Consejo Superior de Investigaciones Científicas, Avda. Montañana 1005, 50059, Zaragoza, Spain aut Vallés M. Departamento de Genética y Producción Vegetal, Estación Experimental de Aula Dei, Consejo Superior de Investigaciones Científicas, Avda. Montañana 1005, 50059, Zaragoza, Spain aut Sanz J. Departamento de Genética y Producción Vegetal, Estación Experimental de Aula Dei, Consejo Superior de Investigaciones Científicas, Avda. Montañana 1005, 50059, Zaragoza, Spain aut Echávarri B. Departamento de Genética y Producción Vegetal, Estación Experimental de Aula Dei, Consejo Superior de Investigaciones Científicas, Avda. Montañana 1005, 50059, Zaragoza, Spain aut Plant Cell Reports Springer-Verlag 25/4(2006-04-01), 257-264 0721-7714 25:4<257 2006 25 299 https://doi.org/10.1007/s00299-005-0047-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0047-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cistué L. Departamento de Genética y Producción Vegetal, Estación Experimental de Aula Dei, Consejo Superior de Investigaciones Científicas, Avda. Montañana 1005, 50059, Zaragoza, Spain aut NATIONALLICENCE 700 1- Soriano M. Departamento de Genética y Producción Vegetal, Estación Experimental de Aula Dei, Consejo Superior de Investigaciones Científicas, Avda. Montañana 1005, 50059, Zaragoza, Spain aut NATIONALLICENCE 700 1- Castillo A. Departamento de Genética y Producción Vegetal, Estación Experimental de Aula Dei, Consejo Superior de Investigaciones Científicas, Avda. Montañana 1005, 50059, Zaragoza, Spain aut NATIONALLICENCE 700 1- Vallés M. Departamento de Genética y Producción Vegetal, Estación Experimental de Aula Dei, Consejo Superior de Investigaciones Científicas, Avda. Montañana 1005, 50059, Zaragoza, Spain aut NATIONALLICENCE 700 1- Sanz J. Departamento de Genética y Producción Vegetal, Estación Experimental de Aula Dei, Consejo Superior de Investigaciones Científicas, Avda. Montañana 1005, 50059, Zaragoza, Spain aut NATIONALLICENCE 700 1- Echávarri B. Departamento de Genética y Producción Vegetal, Estación Experimental de Aula Dei, Consejo Superior de Investigaciones Científicas, Avda. Montañana 1005, 50059, Zaragoza, Spain aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/4(2006-04-01), 257-264 0721-7714 25:4<257 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer