caa a22 4500 467900930 CHVBK 20180406152818.0 cr unu---uuuuu 170328e20060401xx s 000 0 eng 10.1007/s00299-005-0059-4 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0059-4 A model wheat cultivar for transformation to improve resistance to Fusarium Head Blight [Elektronische Daten] [Caroline Mackintosh, David Garvin, Lorien Radmer, Shane Heinen, Gary Muehlbauer] Fusarium head blight (FHB), caused primarily by Fusarium graminearum, is a major disease problem in wheat (Triticum aestivum). Genetic engineering holds significant potential to enhance FHB resistance in wheat. Due to the requirement of screening for FHB resistance on flowers at anthesis, the number of screens carried out in a year is limited. Our objective was to evaluate the feasibility of using the rapid-maturing dwarf wheat cultivar Apogee as an alternative genotype for transgenic FHB resistance research. Our transformation efficiency (number of transgenic plants/number of embryos) for Apogee was 1.33%. Apogee was also found to exhibit high FHB susceptibility and reached anthesis within 4 weeks. Interestingly, microsatellite marker haplotype analysis of the chromosome 3BS FHB resistant quantitative trait locus (QTL) region indicated that this region maybe deleted in Apogee. Our results indicate that Apogee is particularly well suited for accelerating transgenic FHB resistance research and transgenic wheat research in general. Springer-Verlag, 2005 Apogee nationallicence Fusarium graminearum nationallicence Fusarium head blight nationallicence Transgenic wheat nationallicence Triticum aestivum nationallicence FHB : Fusarium head blight nationallicence 2,4-D : 2,4-diclorophenoxyacetic acid nationallicence GUS : β-glucuronidase nationallicence QTL : quantitative trait locus nationallicence Mackintosh Caroline USDA-ARS Plant Science Research Unit and Department of Agronomy and Plant Genetics, University of Minnesota, 411 Borlaug Hall, 1991 Upper Buford Circle, 55108, St Paul, MN, USA aut Garvin David USDA-ARS Plant Science Research Unit and Department of Agronomy and Plant Genetics, University of Minnesota, 411 Borlaug Hall, 1991 Upper Buford Circle, 55108, St Paul, MN, USA aut Radmer Lorien USDA-ARS Plant Science Research Unit and Department of Agronomy and Plant Genetics, University of Minnesota, 411 Borlaug Hall, 1991 Upper Buford Circle, 55108, St Paul, MN, USA aut Heinen Shane USDA-ARS Plant Science Research Unit and Department of Agronomy and Plant Genetics, University of Minnesota, 411 Borlaug Hall, 1991 Upper Buford Circle, 55108, St Paul, MN, USA aut Muehlbauer Gary USDA-ARS Plant Science Research Unit and Department of Agronomy and Plant Genetics, University of Minnesota, 411 Borlaug Hall, 1991 Upper Buford Circle, 55108, St Paul, MN, USA aut Plant Cell Reports Springer-Verlag 25/4(2006-04-01), 313-319 0721-7714 25:4<313 2006 25 299 https://doi.org/10.1007/s00299-005-0059-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0059-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mackintosh Caroline USDA-ARS Plant Science Research Unit and Department of Agronomy and Plant Genetics, University of Minnesota, 411 Borlaug Hall, 1991 Upper Buford Circle, 55108, St Paul, MN, USA aut NATIONALLICENCE 700 1- Garvin David USDA-ARS Plant Science Research Unit and Department of Agronomy and Plant Genetics, University of Minnesota, 411 Borlaug Hall, 1991 Upper Buford Circle, 55108, St Paul, MN, USA aut NATIONALLICENCE 700 1- Radmer Lorien USDA-ARS Plant Science Research Unit and Department of Agronomy and Plant Genetics, University of Minnesota, 411 Borlaug Hall, 1991 Upper Buford Circle, 55108, St Paul, MN, USA aut NATIONALLICENCE 700 1- Heinen Shane USDA-ARS Plant Science Research Unit and Department of Agronomy and Plant Genetics, University of Minnesota, 411 Borlaug Hall, 1991 Upper Buford Circle, 55108, St Paul, MN, USA aut NATIONALLICENCE 700 1- Muehlbauer Gary USDA-ARS Plant Science Research Unit and Department of Agronomy and Plant Genetics, University of Minnesota, 411 Borlaug Hall, 1991 Upper Buford Circle, 55108, St Paul, MN, USA aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/4(2006-04-01), 313-319 0721-7714 25:4<313 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer