caa a22 4500 467900949 CHVBK 20180406152818.0 cr unu---uuuuu 170328e20060401xx s 000 0 eng 10.1007/s00299-005-0074-5 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0074-5 High expression of foot-and-mouth disease virus structural protein VP1 in tobacco chloroplasts [Elektronische Daten] [Yinü Li, Meng Sun, Jixing Liu, Zongqi Yang, Zhifang Zhang, Guifang Shen] A tobacco chloroplast expression vector, pTRVP1, containing the foot-and-mouth disease virus (FMDV) VP1 gene and the selective marker aadA gene, was constructed and transferred to tobacco by biolistic method. Three resistant lines were obtained through spectinomycin selection, and each transgenic line was subjected to a second round of spectinomycin selection. PCR and PCR southern blot analysis revealed that the VP1 gene had integrated into the chloroplast genome. Western blot and quantification ELISA assays indicated that the VP1 gene was expressed in tobacco chloroplasts and accounted for 2-3% of total soluble protein. This suggested that plant chloroplasts were an efficient expression system for the potential production of recombinant antigens in plants. Springer-Verlag, 2005 Foot-and-mouth disease virus (FMDV) nationallicence VP1 protein nationallicence Tobacco transformation nationallicence Chloroplast nationallicence FMDV : Foot-and-mouth disease virus nationallicence CTB : Cholera toxin B submit nationallicence P16S : 16S promoter nationallicence TpsbA : PsbA terminator nationallicence aadA : Aminoglycoside 3'-adenyltransferase nationallicence Spc : Spectinomycin nationallicence 6-BA : N6-benzyladenine nationallicence IAA : Indole-3-acetic acid nationallicence BCIP/NBT : 5-Bromo-4-chloro-3-inoloyl-phosphate/Nitro blue tetrazolium nationallicence TSP : Total soluble protein nationallicence Li Yinü Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 100081, Beijing, P.R. China aut Sun Meng Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 100081, Beijing, P.R. China aut Liu Jixing Lan Zhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046, Lanzhou, P.R. China aut Yang Zongqi Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 100081, Beijing, P.R. China aut Zhang Zhifang Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 100081, Beijing, P.R. China aut Shen Guifang Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 100081, Beijing, P.R. China aut Plant Cell Reports Springer-Verlag 25/4(2006-04-01), 329-333 0721-7714 25:4<329 2006 25 299 https://doi.org/10.1007/s00299-005-0074-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0074-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Yinü Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 100081, Beijing, P.R. China aut NATIONALLICENCE 700 1- Sun Meng Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 100081, Beijing, P.R. China aut NATIONALLICENCE 700 1- Liu Jixing Lan Zhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046, Lanzhou, P.R. China aut NATIONALLICENCE 700 1- Yang Zongqi Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 100081, Beijing, P.R. China aut NATIONALLICENCE 700 1- Zhang Zhifang Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 100081, Beijing, P.R. China aut NATIONALLICENCE 700 1- Shen Guifang Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 100081, Beijing, P.R. China aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/4(2006-04-01), 329-333 0721-7714 25:4<329 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer