caa a22 4500 467900981 CHVBK 20180406152818.0 cr unu---uuuuu 170328e20060401xx s 000 0 eng 10.1007/s00299-005-0053-x doi (NATIONALLICENCE)springer-10.1007/s00299-005-0053-x Genetic transformation of cowpea ( Vigna unguiculata L.) and stable transmission of the transgenes to progeny [Elektronische Daten] [J. Popelka, Stephanie Gollasch, Andy Moore, Lisa Molvig, Thomas Higgins] Cowpeas are nutritious grains that provide the main source of protein, highly digestible energy and vitamins to some of the world's poorest people. The demand for cowpeas is high but yields remain critically low, largely because of insect pests. Cowpea germplasm contains little or no resistance to major insect pests and a gene technology approach to adding insect protection traits is now a high priority. We have adapted features of several legume and other transformation systems and reproducibly obtained transgenic cowpeas that obey Mendelian rules in transmitting the transgene to their progeny. Critical parameters in this transformation system include the choice of cotyledonary nodes from developing or mature seeds as explants and a tissue culture medium devoid of auxins in the early stages, but including the cytokinin BAP at low levels during shoot initiation and elongation. Addition of thiol-compounds during infection and co-culture with Agrobacterium and the choice of the bar gene for selection with phosphinothricin were also important. Transgenic cowpeas that transmit the transgenes to their progeny can be recovered at a rate of one fertile plant per thousand explants. These results pave the way for the introduction of new traits into cowpea and the first genes to be trialled will include those with potential to protect against insect pests. Springer-Verlag, 2005 Agrobacterium- mediated transformation of cowpea nationallicence in vitro culture of cowpea nationallicence Transgenic cowpea nationallicence PAT : phosphinothricin acetyl transferase nationallicence PPT : phosphinothricin nationallicence BAP : benzylaminopurine nationallicence GUS : β-glucuronidase nationallicence Popelka J. CSIRO Plant Industry, GPO Box 1600, ACT 2601, Canberra, Australia aut Gollasch Stephanie CSIRO Plant Industry, GPO Box 1600, ACT 2601, Canberra, Australia aut Moore Andy CSIRO Plant Industry, GPO Box 1600, ACT 2601, Canberra, Australia aut Molvig Lisa CSIRO Plant Industry, GPO Box 1600, ACT 2601, Canberra, Australia aut Higgins Thomas CSIRO Plant Industry, GPO Box 1600, ACT 2601, Canberra, Australia aut Plant Cell Reports Springer-Verlag 25/4(2006-04-01), 304-312 0721-7714 25:4<304 2006 25 299 https://doi.org/10.1007/s00299-005-0053-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0053-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Popelka J. CSIRO Plant Industry, GPO Box 1600, ACT 2601, Canberra, Australia aut NATIONALLICENCE 700 1- Gollasch Stephanie CSIRO Plant Industry, GPO Box 1600, ACT 2601, Canberra, Australia aut NATIONALLICENCE 700 1- Moore Andy CSIRO Plant Industry, GPO Box 1600, ACT 2601, Canberra, Australia aut NATIONALLICENCE 700 1- Molvig Lisa CSIRO Plant Industry, GPO Box 1600, ACT 2601, Canberra, Australia aut NATIONALLICENCE 700 1- Higgins Thomas CSIRO Plant Industry, GPO Box 1600, ACT 2601, Canberra, Australia aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/4(2006-04-01), 304-312 0721-7714 25:4<304 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer