caa a22 4500 46790099X CHVBK 20180406152818.0 cr unu---uuuuu 170328e20060401xx s 000 0 eng 10.1007/s00299-005-0085-2 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0085-2 Somatic embryogenesis and plant regeneration from different wild diploid cotton ( Gossypium ) species [Elektronische Daten] [Yuqiang Sun, Xianlong Zhang, Chao Huang, Xiaoping Guo, Yichun Nie] Calli were successfully induced from hypocotyls of eight wild diploid cotton species (Gossypium) on MSB (MS salts and B5 vitamins) medium supplemented with 0.09μM 2,4-D (2,4-dichlorophenoxyacetic acid) and 2.32μM KT (kinetin). Plant growth regulator (PGR) combinations, adding GA3 (Gibberellic acid), high inorganic salt stress, and PGR-free media were used to induce embryogenic calli from nonembryogenic calli. Embryogenic cultures were induced from G. aridum S. (D4 genome), G. davidsonii K. (D3-d genome), G. klotzschianum A. (D3-k genome), G. raimondii U. (D5 genome), and G. stocksii M. (E1 genome). We then observed somatic embryogenesis in the five species while calli of G. africanum V. (A1-2 genome), G. anomalum W. (B1 genome), and G. bickii P. (G genome) remained nonembryogenic. Somatic embryogenesis was adjusted by changing sugar sources, regulating combinations of PGRs, and using cell suspension culture. Embryos at various developmental stages produced mature and germinating embryos when cultured on filter paper placed on the media containing different sugar sources. The utility of different sugar sources promoted globular embryos developing into cotyledonary stage and increased the frequency of cotyledonary embryos developing into normal plants. Normal plantlets were regenerated from G. davidsonii, G. klotzschianum, G. raimondii, and G. stocksii. Only abnormal plantlets were obtained in G. aridum. This work will contribute to broadening the number of regenerable cotton species and provide foundations for somatic hybridization in cotton to create new germplasm. Springer-Verlag, 2005 Differentiation nationallicence Germplasm nationallicence Gossypium nationallicence Plant growth regulation nationallicence Plant regeneration nationallicence Wild diploid cotton nationallicence Sun Yuqiang National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P.R. China aut Zhang Xianlong National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P.R. China aut Huang Chao National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P.R. China aut Guo Xiaoping National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P.R. China aut Nie Yichun National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P.R. China aut Plant Cell Reports Springer-Verlag 25/4(2006-04-01), 289-296 0721-7714 25:4<289 2006 25 299 https://doi.org/10.1007/s00299-005-0085-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0085-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sun Yuqiang National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P.R. China aut NATIONALLICENCE 700 1- Zhang Xianlong National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P.R. China aut NATIONALLICENCE 700 1- Huang Chao National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P.R. China aut NATIONALLICENCE 700 1- Guo Xiaoping National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P.R. China aut NATIONALLICENCE 700 1- Nie Yichun National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P.R. China aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/4(2006-04-01), 289-296 0721-7714 25:4<289 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer