caa a22 4500 467901007 CHVBK 20180406152818.0 cr unu---uuuuu 170328e20060401xx s 000 0 eng 10.1007/s00299-005-0058-5 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0058-5 Agrobacterium -mediated transformation of seedling-derived maize callus [Elektronische Daten] [Vladimir Sidorov, Larry Gilbertson, Prince Addae, David Duncan] Efficient production of seedling-derived Type I callus was demonstrated for several corn genotypes including commercial inbred lines. Seeds were germinated on MS-based medium containing 10mgl−1 picloram and 3mgl−1 6-benzylaminopurine, which induced the development of axillary buds in the area of coleoptilar node. Nodal sections of 7-10-day old seedlings were isolated, split longitudinally, and placed on callus induction medium supplemented with 2.2mgl−1 picloram and 0.5mgl−1 2,4-dichlorophenoxyacetic acid. For lines L4 and L9 the frequency of embryogenic callus induction was 38-42% based on calli per split nodal section. Frequency of callus induction from split nodal sections of seeds germinated on media without growth regulators was 0-3%. Seedling-derived callus of five genotypes was used for Agrobacterium-mediated transformation. Two constructs containing the green fluorescence protein gene and genes for either neomycin phosphotransferase II or glyphosate selection were used in transformation experiments. Transformation frequency varied from 2 to 11% and about 60% of the T0 plants had 1-2 copies of transgenes. Springer-Verlag, 2005 Maize nationallicence Seedling-derived callus nationallicence Agrobacterium transformation nationallicence Sidorov Vladimir Monsanto Company, 700 Chesterfield Parkway North, 63017, St. Louis, MO, USA aut Gilbertson Larry Monsanto Company, 700 Chesterfield Parkway North, 63017, St. Louis, MO, USA aut Addae Prince Monsanto Company, 700 Chesterfield Parkway North, 63017, St. Louis, MO, USA aut Duncan David Monsanto Company, 700 Chesterfield Parkway North, 63017, St. Louis, MO, USA aut Plant Cell Reports Springer-Verlag 25/4(2006-04-01), 320-328 0721-7714 25:4<320 2006 25 299 https://doi.org/10.1007/s00299-005-0058-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0058-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sidorov Vladimir Monsanto Company, 700 Chesterfield Parkway North, 63017, St. Louis, MO, USA aut NATIONALLICENCE 700 1- Gilbertson Larry Monsanto Company, 700 Chesterfield Parkway North, 63017, St. Louis, MO, USA aut NATIONALLICENCE 700 1- Addae Prince Monsanto Company, 700 Chesterfield Parkway North, 63017, St. Louis, MO, USA aut NATIONALLICENCE 700 1- Duncan David Monsanto Company, 700 Chesterfield Parkway North, 63017, St. Louis, MO, USA aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/4(2006-04-01), 320-328 0721-7714 25:4<320 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer