caa a22 4500 467901015 CHVBK 20180406152818.0 cr unu---uuuuu 170328e20060401xx s 000 0 eng 10.1007/s00299-005-0086-1 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0086-1 Targeted overexpression of the Escherichia coli MinC protein in higher plants results in abnormal chloroplasts [Elektronische Daten] [Venkata Tavva, Glenn Collins, Randy Dinkins] Higher plant chloroplast division involves some of the same types of proteins that are required in prokaryotic cell division. These include two of the three Min proteins, MinD and MinE, encoded by the min operon in bacteria. Noticeably absent from annotated sequences from higher plants is a MinC homologue. A higher plant functional MinC homologue that would interfere with FtsZ polymerization, has yet to be identified. We sought to determine whether expression of the bacterial MinC in higher plants could affect chloroplast division. The Escherichia coli minC (EcMinC) gene was isolated and inserted behind the Arabidopsis thaliana RbcS transit peptide sequence for chloroplast targeting. This TP-EcMinC gene driven by the CaMV 35S2 constitutive promoter was then transformed into tobacco (Nicotiana tabacum L.). Abnormally large chloroplasts were observed in the transgenic plants suggesting that overexpression of the E. coli MinC perturbed higher plant chloroplast division. Springer-Verlag, 2005 Chloroplast division nationallicence Min proteins nationallicence Transgenic plants nationallicence Escherichia coli nationallicence Tavva Venkata Department of Plant and Soil Sciences, University of Kentucky, 1405 Veterans Road, 40546-0312, Lexington, KY, USA aut Collins Glenn Department of Plant and Soil Sciences, University of Kentucky, 1405 Veterans Road, 40546-0312, Lexington, KY, USA aut Dinkins Randy Department of Plant and Soil Sciences, University of Kentucky, 1405 Veterans Road, 40546-0312, Lexington, KY, USA aut Plant Cell Reports Springer-Verlag 25/4(2006-04-01), 341-348 0721-7714 25:4<341 2006 25 299 https://doi.org/10.1007/s00299-005-0086-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0086-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tavva Venkata Department of Plant and Soil Sciences, University of Kentucky, 1405 Veterans Road, 40546-0312, Lexington, KY, USA aut NATIONALLICENCE 700 1- Collins Glenn Department of Plant and Soil Sciences, University of Kentucky, 1405 Veterans Road, 40546-0312, Lexington, KY, USA aut NATIONALLICENCE 700 1- Dinkins Randy Department of Plant and Soil Sciences, University of Kentucky, 1405 Veterans Road, 40546-0312, Lexington, KY, USA aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/4(2006-04-01), 341-348 0721-7714 25:4<341 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer