caa a22 4500 467901031 CHVBK 20180406152818.0 cr unu---uuuuu 170328e20060401xx s 000 0 eng 10.1007/s00299-005-0052-y doi (NATIONALLICENCE)springer-10.1007/s00299-005-0052-y Expression of sarcotoxin IA gene via a root-specific tob promoter enhanced host resistance against parasitic weeds in tomato plants [Elektronische Daten] [Aly Radi, Plakhin Dina, Achdari Guy] We have developed a simple genetic engineering strategy for conferring resistance against parasitic weeds on host plants. Transgenic tomato plants expressing the sarcotoxin IA gene were grown either in polyethylene bags (PE) or in pots inoculated with Orobanche aegyptiaca seeds. The results indicate that transgenic plants exhibited strong inhibition of parasite growth and significantly increased yield as compared with non-transgenic ones. In both PE and pot systems most of the parasite tubercles attached to the transgenic root plants turned necrotic and developed abnormally. Integration and expression of the gene were confirmed by Southern blot, RT-PCR and Western blot analysis. Our results indicate that the insect gene produced in the plant cells was selectively toxic to the parasite and non-toxic to the host plant. Springer-Verlag, 2005 Orobanche aegyptiaca nationallicence Parasitic weeds nationallicence Sarcotoxin IA nationallicence Transgenic tomato nationallicence Radi Aly Agricultural Research Organization (ARO), Department of Weed Research, Newe Yaar Research Center, Ramat Yishai, 30095, Ramat, Israel aut Dina Plakhin Agricultural Research Organization (ARO), Department of Weed Research, Newe Yaar Research Center, Ramat Yishai, 30095, Ramat, Israel aut Guy Achdari Agricultural Research Organization (ARO), Department of Weed Research, Newe Yaar Research Center, Ramat Yishai, 30095, Ramat, Israel aut Plant Cell Reports Springer-Verlag 25/4(2006-04-01), 297-303 0721-7714 25:4<297 2006 25 299 https://doi.org/10.1007/s00299-005-0052-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0052-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Radi Aly Agricultural Research Organization (ARO), Department of Weed Research, Newe Yaar Research Center, Ramat Yishai, 30095, Ramat, Israel aut NATIONALLICENCE 700 1- Dina Plakhin Agricultural Research Organization (ARO), Department of Weed Research, Newe Yaar Research Center, Ramat Yishai, 30095, Ramat, Israel aut NATIONALLICENCE 700 1- Guy Achdari Agricultural Research Organization (ARO), Department of Weed Research, Newe Yaar Research Center, Ramat Yishai, 30095, Ramat, Israel aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/4(2006-04-01), 297-303 0721-7714 25:4<297 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer