caa a22 4500 467901074 CHVBK 20180406152818.0 cr unu---uuuuu 170328e20060801xx s 000 0 eng 10.1007/s00299-006-0123-8 doi (NATIONALLICENCE)springer-10.1007/s00299-006-0123-8 Transgenic zoysiagrass ( Zoysia japonica ) plants obtained by Agrobacterium -mediated transformation [Elektronische Daten] [Yaxin Ge, Tina Norton, Zeng-Yu Wang] Zoysiagrass (Zoysia japonica Steud.) is an important turfgrass that spreads by stolons and rhizomes. By exploring the potential of direct shoot formation from stolons, we developed a straightforward and efficient transformation protocol without callus induction and propagation. Sterilized stolon nodes were infected and co-cultivated with Agrobacterium tumefaciens harboring pCAMBIA vectors. Hygromycin phosphotransferase gene (hph) was used as the selectable marker and hygromycin was used as the selection agent. Both green and albino shoots were directly regenerated from the infected stolon nodes 4-5 weeks after hygromycin selection. Greenhouse-grown plants were obtained 10-12 weeks after Agrobacterium-mediated transformation. Based on the number of transgenic plants obtained and the number of stolon nodes infected, a transformation frequency of 6.8% was achieved. Stable integration of the transgenes in the plant genome was demonstrated by PCR and Southern blot hybridization analyses. Expression of the transgenes was confirmed by RT-PCR analysis and GUS staining. The new transformation system opens up new opportunities for the functional characterization of genes and promoters and the development of novel germplasm in zoysiagrass. Springer-Verlag, 2006 Agrobacterium nationallicence Genetic transformation nationallicence Transgenic plant nationallicence Turfgrass nationallicence Zoysia japonica nationallicence Zoysiagrass nationallicence Ge Yaxin Forage Improvement Division, The Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA aut Norton Tina Forage Improvement Division, The Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA aut Wang Zeng-Yu Forage Improvement Division, The Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA aut Plant Cell Reports Springer-Verlag 25/8(2006-08-01), 792-798 0721-7714 25:8<792 2006 25 299 https://doi.org/10.1007/s00299-006-0123-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0123-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ge Yaxin Forage Improvement Division, The Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA aut NATIONALLICENCE 700 1- Norton Tina Forage Improvement Division, The Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA aut NATIONALLICENCE 700 1- Wang Zeng-Yu Forage Improvement Division, The Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/8(2006-08-01), 792-798 0721-7714 25:8<792 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer