caa a22 4500 467901090 CHVBK 20180406152818.0 cr unu---uuuuu 170328e20060801xx s 000 0 eng 10.1007/s00299-006-0117-6 doi (NATIONALLICENCE)springer-10.1007/s00299-006-0117-6 Strategies to obtain stable transgenic plants from non-embryogenic lines: complementation of the nn 1 mutation of the NORK gene in Medicago sativa MN1008 [Elektronische Daten] [Ariana Perhald, Gabriella Endre, Zoltan Kevei, Gyorgy Kiss, Attila Kereszt] Strategies to introduce genes into non-embryogenic plants for complementation of a mutation are described and tested on tetraploid alfalfa (Medicago sativa). Genes conditioning embryogenic potential, a mutant phenotype, and a gene to complement the mutation can be combined using several different crossing and selection steps. In the successful strategy used here, the M. sativa genotype MnNC-1008(NN) carrying the recessive non-nodulating mutant allele nn 1 was crossed with the highly embryogenic alfalfa line Regen S and embryogenic hybrid individuals were identified from the F1 progeny. After transformation of these hybrids with the wild-type gene (NORK), an F2 generation segregating for the mutation and transgene were produced. Plants homozygous for the mutant allele and carrying the wild-type NORK transgene could form root nodules after inoculation with Sinorhizobium meliloti demonstrating successful complementation of the nn 1 mutation. Springer-Verlag, 2006 Medicago nationallicence Stable transformation nationallicence Embryogenicity nationallicence Nodulation nationallicence Mutant nationallicence Complementation nationallicence Perhald Ariana Institute of Genetics, Biological Research Center of the Hungarian Academy of Sciences, Temesvari korut 62, 6726, Szeged, Hungary aut Endre Gabriella Institute of Genetics, Biological Research Center of the Hungarian Academy of Sciences, Temesvari korut 62, 6726, Szeged, Hungary aut Kevei Zoltan Institute of Genetics, Biological Research Center of the Hungarian Academy of Sciences, Temesvari korut 62, 6726, Szeged, Hungary aut Kiss Gyorgy Institute of Genetics, Biological Research Center of the Hungarian Academy of Sciences, Temesvari korut 62, 6726, Szeged, Hungary aut Kereszt Attila Institute of Genetics, Biological Research Center of the Hungarian Academy of Sciences, Temesvari korut 62, 6726, Szeged, Hungary aut Plant Cell Reports Springer-Verlag 25/8(2006-08-01), 799-806 0721-7714 25:8<799 2006 25 299 https://doi.org/10.1007/s00299-006-0117-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0117-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Perhald Ariana Institute of Genetics, Biological Research Center of the Hungarian Academy of Sciences, Temesvari korut 62, 6726, Szeged, Hungary aut NATIONALLICENCE 700 1- Endre Gabriella Institute of Genetics, Biological Research Center of the Hungarian Academy of Sciences, Temesvari korut 62, 6726, Szeged, Hungary aut NATIONALLICENCE 700 1- Kevei Zoltan Institute of Genetics, Biological Research Center of the Hungarian Academy of Sciences, Temesvari korut 62, 6726, Szeged, Hungary aut NATIONALLICENCE 700 1- Kiss Gyorgy Institute of Genetics, Biological Research Center of the Hungarian Academy of Sciences, Temesvari korut 62, 6726, Szeged, Hungary aut NATIONALLICENCE 700 1- Kereszt Attila Institute of Genetics, Biological Research Center of the Hungarian Academy of Sciences, Temesvari korut 62, 6726, Szeged, Hungary aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/8(2006-08-01), 799-806 0721-7714 25:8<799 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer