caa a22 4500 467901112 CHVBK 20180406152818.0 cr unu---uuuuu 170328e20060801xx s 000 0 eng 10.1007/s00299-006-0115-8 doi (NATIONALLICENCE)springer-10.1007/s00299-006-0115-8 Simplified and improved somatic embryogenesis for clonal propagation of Pinus pinaster (Ait.) [Elektronische Daten] [Marie-Anne Lelu-Walter, Michele Bernier-Cardou, Krystyna Klimaszewska] In this study, several improvements and simplifications of SE protocols in Pinus pinaster (Ait.), a species of economic importance in the regions of Western Europe, are described. These improvements pertained to all stages of SE including high initiation frequencies in eight control pollinated seed families, relatively high somatic embryo maturation yield when cells were coated with particles of activated charcoal and a rapid production of plants directly in a shade house. The SE initiation frequency from isolated zygotic embryos was high (up to 100%) and plants were produced from 11 embryogenic lines representing all crosses. Based on these results, the estimated number of somatic embryos required to produce 1,000 plants varied from slightly more than the required number of plants to more than double this number depending on the line. Such an estimate is critical in developing plant production strategy when a number of embryogenic lines are considered for production of clonal plants. Springer-Verlag, 2006 Embryonal mass nationallicence Maritime pine nationallicence Plant propagation nationallicence Somatic embryos nationallicence Lelu-Walter Marie-Anne Unité Amélioration, Génétique et Physiologie Forestières, INRA-CRO BP 20 619, Ardon F-45166, Olivet Cedex, France aut Bernier-Cardou Michele Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 rue du P.E.P.S., Sainte-Foy, Quebec, Canada aut Klimaszewska Krystyna Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 rue du P.E.P.S., Sainte-Foy, Quebec, Canada aut Plant Cell Reports Springer-Verlag 25/8(2006-08-01), 767-776 0721-7714 25:8<767 2006 25 299 https://doi.org/10.1007/s00299-006-0115-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0115-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lelu-Walter Marie-Anne Unité Amélioration, Génétique et Physiologie Forestières, INRA-CRO BP 20 619, Ardon F-45166, Olivet Cedex, France aut NATIONALLICENCE 700 1- Bernier-Cardou Michele Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 rue du P.E.P.S., Sainte-Foy, Quebec, Canada aut NATIONALLICENCE 700 1- Klimaszewska Krystyna Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 rue du P.E.P.S., Sainte-Foy, Quebec, Canada aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/8(2006-08-01), 767-776 0721-7714 25:8<767 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer