caa a22 4500 467901139 CHVBK 20180406152818.0 cr unu---uuuuu 170328e20060801xx s 000 0 eng 10.1007/s00299-005-0076-3 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0076-3 Two promoters conferring active gene expression in vegetative nuclei of tobacco immature pollen undergoing embryogenic dedifferentiation [Elektronische Daten] [Naoki Yamaji, Masaharu Kyo] In order to visualize the specific state of tobacco pollen undergoing dedifferentiation from immature pollen to embryogenic cells, we established tobacco marker lines transgenic for a vital reporter gene regulated under the transcriptional control of an 840bp fragment, named A22pro. This fragment was obtained from the 5′-flanking region of a gene corresponding to a cDNA named A22 that was previously isolated through differential screening from a cDNA library prepared from tobacco pollen undergoing dedifferentiation. The reporter gene, named H3sGFP, consisting of synthetic green fluorescent protein gene (sGFP) and tobacco H3 histone gene for nuclear localization, was designed to distinguish the gene expression in the generative cell from that in the vegetative cell in a pollen grain. The marker line produced pollen showing a green fluorescent signal in the generative nuclei (GN) but the expression level of the transgene was low. Pollen after culture for dedifferentiation showed an intense signal transiently in the vegetative nuclei (VN), at a specific developmental stage of pollen, with a rapid increase of expression level of the transgene. Serial observations revealed that all androgenic embryos originated from the pollen grains that had shown the signal in their VN. Thus, A22pro is originally functional in gametogenesis but is activated in VN of pollen undergoing embryogenic dedifferentiation. Additionally, we observed a gene expression pattern identical to that described above, using another 5′-flanking region of a gene for a cDNA, named B27pro, homologous to A22 as a promoter of the reporter gene. Springer-Verlag, 2005 Dedifferentiation nationallicence GFP nationallicence Marker line nationallicence Nicotiana tabacum nationallicence Pollen embryogenesis nationallicence Promoter nationallicence Yamaji Naoki Department of Life Sciences, Faculty of Agriculture, Kagawa University, 761-0795, Miki, Kagawa, Japan aut Kyo Masaharu Department of Life Sciences, Faculty of Agriculture, Kagawa University, 761-0795, Miki, Kagawa, Japan aut Plant Cell Reports Springer-Verlag 25/8(2006-08-01), 749-757 0721-7714 25:8<749 2006 25 299 https://doi.org/10.1007/s00299-005-0076-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0076-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yamaji Naoki Department of Life Sciences, Faculty of Agriculture, Kagawa University, 761-0795, Miki, Kagawa, Japan aut NATIONALLICENCE 700 1- Kyo Masaharu Department of Life Sciences, Faculty of Agriculture, Kagawa University, 761-0795, Miki, Kagawa, Japan aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/8(2006-08-01), 749-757 0721-7714 25:8<749 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer