caa a22 4500 467901147 CHVBK 20180406152818.0 cr unu---uuuuu 170328e20060801xx s 000 0 eng 10.1007/s00299-006-0128-3 doi (NATIONALLICENCE)springer-10.1007/s00299-006-0128-3 Characterization of a novel glycine-rich protein from the cell wall of maize silk tissues [Elektronische Daten] [T. Tao, T. Ouellet, K. Dadej, S. Miller, D. Johnson, J. Singh] The isolation, characterization and regulation of expression of a maize silk-specific gene is described. zmgrp5 (Zea mays glycine-rich protein 5) encodes a 187 amino acid glycine-rich protein that displays developmentally regulated silk-specific expression. Northern, Western, in situ mRNA hybridization and transient gene expression analyses indicate that zmgrp5 is expressed in silk hair and in cells of the vascular bundle and pollen tube transmitting tissue elements. The protein is secreted into the extracellular matrix and is localized in the cell wall fraction mainly through interactions mediated by covalent disulphide bridges. Taken together, these results suggest that the protein may play a role in maintaining silk structure during development. This is the first documented isolation of a stigma-specific gene from maize, an important agronomic member of the Poaceae family. Springer-Verlag, 2006 Glycine-rich protein nationallicence Silk nationallicence Silk-hair nationallicence Stigma nationallicence zmgrp5 nationallicence Tao T. Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, K.W. Neatby Bldg., Room 2091, K1A 0C6, Ottawa, Ontario, Canada aut Ouellet T. Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, K.W. Neatby Bldg., Room 2091, K1A 0C6, Ottawa, Ontario, Canada aut Dadej K. Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, K.W. Neatby Bldg., Room 2091, K1A 0C6, Ottawa, Ontario, Canada aut Miller S. Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, K.W. Neatby Bldg., Room 2091, K1A 0C6, Ottawa, Ontario, Canada aut Johnson D. Department of Biology, University of Ottawa, 30 Marie Curie, K1N 6N5, P.O. Box 450, Station A, Ottawa, Ontario, Canada aut Singh J. Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, K.W. Neatby Bldg., Room 2091, K1A 0C6, Ottawa, Ontario, Canada aut Plant Cell Reports Springer-Verlag 25/8(2006-08-01), 848-858 0721-7714 25:8<848 2006 25 299 https://doi.org/10.1007/s00299-006-0128-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0128-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tao T. Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, K.W. Neatby Bldg., Room 2091, K1A 0C6, Ottawa, Ontario, Canada aut NATIONALLICENCE 700 1- Ouellet T. Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, K.W. Neatby Bldg., Room 2091, K1A 0C6, Ottawa, Ontario, Canada aut NATIONALLICENCE 700 1- Dadej K. Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, K.W. Neatby Bldg., Room 2091, K1A 0C6, Ottawa, Ontario, Canada aut NATIONALLICENCE 700 1- Miller S. Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, K.W. Neatby Bldg., Room 2091, K1A 0C6, Ottawa, Ontario, Canada aut NATIONALLICENCE 700 1- Johnson D. Department of Biology, University of Ottawa, 30 Marie Curie, K1N 6N5, P.O. Box 450, Station A, Ottawa, Ontario, Canada aut NATIONALLICENCE 700 1- Singh J. Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, K.W. Neatby Bldg., Room 2091, K1A 0C6, Ottawa, Ontario, Canada aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/8(2006-08-01), 848-858 0721-7714 25:8<848 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer