caa a22 4500 467901155 CHVBK 20180406152818.0 cr unu---uuuuu 170328e20060801xx s 000 0 eng 10.1007/s00299-006-0136-3 doi (NATIONALLICENCE)springer-10.1007/s00299-006-0136-3 Cloning and characterization of 2- C -methyl- d -erythritol 2,4-cyclodiphosphate synthase (MECS) gene from Ginkgo biloba [Elektronische Daten] [Sang-Min Kim, Tomohisa Kuzuyama, Yung-Jin Chang, Soo-Un Kim] Ginkgo biloba contains secondary metabolites with interesting pharmacological properties, including highly modified diterpenoid ginkgolide, potent and selective antagonist of platelet-activating factor. 2-C-Methyl-d-erythritol 2,4-cyclodiphosphate synthase gene (GbMECS) involved in ginkgolide biosynthesis pathway was cloned and characterized from G. biloba embryonic roots, and the full open reading frame was deduced as protein consisting of 238 amino acid residues. Putative mature protein with a 179 residue-long sequence, obtained by deleting N-terminal chloroplast transit peptide region composed of 59 amino acid residues, rescued Esherichia coli NMW26, an E. coli knock-out mutant of ygbB (EcMECS). Transcription levels of GbMECS were two-fold higher in embryo roots compared to leaves. When full-length GbMECS with chloroplast transit peptide sequence was fused to green fluorescent protein gene (GFP), and transiently expressed in Arabidopsis thaliana protoplast, green fluorescence was found in chloroplast, indication of protein transportation into plastid. Springer-Verlag, 2006 Bilobalide nationallicence 2- C -Methyl- d -erythritol 2,4-cyclodiphosphate synthase nationallicence Ginkgo biloba nationallicence Ginkgolide nationallicence Protoplast nationallicence Kim Sang-Min Program in Applied Life Chemistry, School of Agricultural Biotechnology, Seoul National University, 151-921, Seoul, Korea aut Kuzuyama Tomohisa Laboratory of Cell Biotechnology, Biotechnology Research Center, University of Tokyo, 113-8657, Tokyo, Japan aut Chang Yung-Jin Program in Applied Life Chemistry, School of Agricultural Biotechnology, Seoul National University, 151-921, Seoul, Korea aut Kim Soo-Un Program in Applied Life Chemistry, School of Agricultural Biotechnology, Seoul National University, 151-921, Seoul, Korea aut Plant Cell Reports Springer-Verlag 25/8(2006-08-01), 829-835 0721-7714 25:8<829 2006 25 299 https://doi.org/10.1007/s00299-006-0136-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0136-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kim Sang-Min Program in Applied Life Chemistry, School of Agricultural Biotechnology, Seoul National University, 151-921, Seoul, Korea aut NATIONALLICENCE 700 1- Kuzuyama Tomohisa Laboratory of Cell Biotechnology, Biotechnology Research Center, University of Tokyo, 113-8657, Tokyo, Japan aut NATIONALLICENCE 700 1- Chang Yung-Jin Program in Applied Life Chemistry, School of Agricultural Biotechnology, Seoul National University, 151-921, Seoul, Korea aut NATIONALLICENCE 700 1- Kim Soo-Un Program in Applied Life Chemistry, School of Agricultural Biotechnology, Seoul National University, 151-921, Seoul, Korea aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/8(2006-08-01), 829-835 0721-7714 25:8<829 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer