caa a22 4500 467901171 CHVBK 20180406152818.0 cr unu---uuuuu 170328e20060801xx s 000 0 eng 10.1007/s00299-006-0140-7 doi (NATIONALLICENCE)springer-10.1007/s00299-006-0140-7 Molecular analysis of transgene and vector backbone integration into the barley genome following Agrobacterium -mediated transformation [Elektronische Daten] [Mette Lange, Eva Vincze, Marianne Møller, Preben Holm] We report a large-scale study on the frequency of transgene and T-DNA backbone integration following Agrobacterium-mediated transformation of immature barley embryos. One hundred and ninety-one plant lines were regenerated after hygromycin selection and visual selection for GFP expression at the callus stage. Southern blotting performed on a subset of 53 lines that were PCR positive for the GFP gene documented the integration of the GFP gene in 27 of the lines. Twenty-three of these lines expressed GFP in T1 plantlets. Southern blotting with a vector backbone probe revealed that 13 of the 27 lines possessed one or more vector backbone fragments illustrating the regular occurrence of vector backbone integration following Agrobacterium infection of barley immature embryos. Springer-Verlag, 2006 Barley transformation nationallicence pVec8-GFP nationallicence T-DNA integration nationallicence Vector backbone integration nationallicence Lange Mette Department of Genetics and Biotechnology, Danish Institute of Agricultural Sciences, Research Centre Flakkebjerg, Forsoegsvej 1, DK-4200, Slagelse, Denmark aut Vincze Eva Department of Genetics and Biotechnology, Danish Institute of Agricultural Sciences, Research Centre Flakkebjerg, Forsoegsvej 1, DK-4200, Slagelse, Denmark aut Møller Marianne Department of Genetics and Biotechnology, Danish Institute of Agricultural Sciences, Research Centre Flakkebjerg, Forsoegsvej 1, DK-4200, Slagelse, Denmark aut Holm Preben Department of Genetics and Biotechnology, Danish Institute of Agricultural Sciences, Research Centre Flakkebjerg, Forsoegsvej 1, DK-4200, Slagelse, Denmark aut Plant Cell Reports Springer-Verlag 25/8(2006-08-01), 815-820 0721-7714 25:8<815 2006 25 299 https://doi.org/10.1007/s00299-006-0140-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0140-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lange Mette Department of Genetics and Biotechnology, Danish Institute of Agricultural Sciences, Research Centre Flakkebjerg, Forsoegsvej 1, DK-4200, Slagelse, Denmark aut NATIONALLICENCE 700 1- Vincze Eva Department of Genetics and Biotechnology, Danish Institute of Agricultural Sciences, Research Centre Flakkebjerg, Forsoegsvej 1, DK-4200, Slagelse, Denmark aut NATIONALLICENCE 700 1- Møller Marianne Department of Genetics and Biotechnology, Danish Institute of Agricultural Sciences, Research Centre Flakkebjerg, Forsoegsvej 1, DK-4200, Slagelse, Denmark aut NATIONALLICENCE 700 1- Holm Preben Department of Genetics and Biotechnology, Danish Institute of Agricultural Sciences, Research Centre Flakkebjerg, Forsoegsvej 1, DK-4200, Slagelse, Denmark aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/8(2006-08-01), 815-820 0721-7714 25:8<815 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer