caa a22 4500 46790121X CHVBK 20180406152818.0 cr unu---uuuuu 170328e20060501xx s 000 0 eng 10.1007/s00299-005-0083-4 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0083-4 Agrobacterium -mediated genetic transformation of the desiccation tolerant resurrection plant Ramonda myconi (L.) Rchb [Elektronische Daten] [Sándor Tóth, Csaba Kiss, Peter Scott, Gabriella Kovács, Seppo Sorvari, Ottó Toldi] In this paper we describe the first procedure for Agrobacterium tumefaciens-mediated genetic transformation of the desiccation tolerant plant Ramonda myconi (L.) Rchb. Previously, we reported the establishment of a reliable and effective tissue culture system based on the integrated optimisation of antioxidant and growth regulator composition and the stabilisation of the pH of the culture media by means of a potassium phosphate buffer. This efficient plant regeneration via callus phase provided a basis for the optimisation of the genetic transformation in R. myconi. For gene delivery, both a standard (method A) and a modified protocol (method B) have been applied. Since the latter has previously resulted in successful transformation of another resurrection plant, Craterostigma plantagineum, an identical protocol was utilized in transformation of R. myconi, as this method may prove general for dicotyledonous resurrection plants. On this basis, physical and biochemical key variables in transformation were evaluated such as mechanical microwounding of plant explants and in vitro preinduction of vir genes. While the physical enhancement of bacterial penetration was proved to be essential for successful genetic transformation of R. myconi, an additional two-fold increase in the transformation frequency was obtained when the above physical and biochemical treatments were applied in combination. All R 0 and R 1 transgenic plants were fertile, and no morphological abnormalities were observed on the whole-plant level. Springer-Verlag, 2005 Agrobacterium tumefaciens nationallicence Desiccation tolerance nationallicence Genetic transformation nationallicence Ramonda myconi nationallicence Resurrection plant nationallicence GM medium : Germination medium (Toldi etal. 1994) nationallicence RA medium : Ramonda medium (Tóth etal. 2004) nationallicence MSI medium : Modified MS medium for bacterial infection (Borsics etal. 2002) nationallicence CPY medium : Culture medium for Agrobacterium nationallicence BAP : 6-Benzyladenine nationallicence NAA : 1-Naphtaleneacetic acid nationallicence sgm explants : Leaf segment explants nationallicence int explants : Intact, but microwounded explants nationallicence Tóth Sándor Agricultural Biotechnology Center, P.O. Box 411, H-2101, Gödöllő, Hungary aut Kiss Csaba Agricultural Biotechnology Center, P.O. Box 411, H-2101, Gödöllő, Hungary aut Scott Peter School of Life Sciences, University of Sussex, BN1 9QG, Falmer, Brighton, UK aut Kovács Gabriella Agricultural Biotechnology Center, P.O. Box 411, H-2101, Gödöllő, Hungary aut Sorvari Seppo MTT Agrifood Research Finland, Institute of Horticulture, Toivonlinnantie 518, FIN-21500, Piikkiö, Finland aut Toldi Ottó Agricultural Biotechnology Center, P.O. Box 411, H-2101, Gödöllő, Hungary aut Plant Cell Reports Springer-Verlag 25/5(2006-05-01), 442-449 0721-7714 25:5<442 2006 25 299 https://doi.org/10.1007/s00299-005-0083-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0083-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tóth Sándor Agricultural Biotechnology Center, P.O. Box 411, H-2101, Gödöllő, Hungary aut NATIONALLICENCE 700 1- Kiss Csaba Agricultural Biotechnology Center, P.O. Box 411, H-2101, Gödöllő, Hungary aut NATIONALLICENCE 700 1- Scott Peter School of Life Sciences, University of Sussex, BN1 9QG, Falmer, Brighton, UK aut NATIONALLICENCE 700 1- Kovács Gabriella Agricultural Biotechnology Center, P.O. Box 411, H-2101, Gödöllő, Hungary aut NATIONALLICENCE 700 1- Sorvari Seppo MTT Agrifood Research Finland, Institute of Horticulture, Toivonlinnantie 518, FIN-21500, Piikkiö, Finland aut NATIONALLICENCE 700 1- Toldi Ottó Agricultural Biotechnology Center, P.O. Box 411, H-2101, Gödöllő, Hungary aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/5(2006-05-01), 442-449 0721-7714 25:5<442 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer