caa a22 4500 467901228 CHVBK 20180406152818.0 cr unu---uuuuu 170328e20060501xx s 000 0 eng 10.1007/s00299-005-0067-4 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0067-4 Methotrexate is a new selectable marker for tobacco immature pollen transformation [Elektronische Daten] [Tatiana Aionesei, Julia Hosp, Viktor Voronin, Erwin Heberle-Bors, Alisher Touraev] We report here a new selectable marker for tobacco immature pollen transformation based on the expression of dihydrofolate reductase (dhfr) gene which confers resistance to methotrexate (Mtx). Two immature pollen transformation approaches, i.e., male germ line transformation and particle bombardment of embryogenic mid-bicellular pollen have been used for the production of stable transgenic tobacco plants. In the first method, two methotrexate-resistant plants were selected from a total of 7161 seeds recovered after transformation experiments. In the second method, four methotrexate-resistant plants were obtained from 29 bombardments using 3.7×105 pollen grains per bombardment. Southern analysis confirmed the transgenic nature of T0 and T1 candidate transgenic plants, and a genetic analysis showed that the transgenes are transmitted to subsequent generations. Springer-Verlag, 2005 Nicotiana tabacum nationallicence Microspore transformation nationallicence Particle bombardment nationallicence Methotrexate nationallicence dhfr : Dihydrofolate reductase nationallicence Mtx: Methotrexate nationallicence PAT: Phosphinothricin acetyltransferase nationallicence CaMV 35S: Cauliflower mosaic virus promoter nationallicence Aionesei Tatiana Max F. Perutz Laboratories at the Vienna Biocenter, Department of Genetics, Vienna University, Dr. Bohrgasse 9/4, A-1030, Vienna, Austria aut Hosp Julia Max F. Perutz Laboratories at the Vienna Biocenter, Department of Genetics, Vienna University, Dr. Bohrgasse 9/4, A-1030, Vienna, Austria aut Voronin Viktor Max F. Perutz Laboratories at the Vienna Biocenter, Department of Genetics, Vienna University, Dr. Bohrgasse 9/4, A-1030, Vienna, Austria aut Heberle-Bors Erwin Max F. Perutz Laboratories at the Vienna Biocenter, Department of Genetics, Vienna University, Dr. Bohrgasse 9/4, A-1030, Vienna, Austria aut Touraev Alisher Max F. Perutz Laboratories at the Vienna Biocenter, Department of Genetics, Vienna University, Dr. Bohrgasse 9/4, A-1030, Vienna, Austria aut Plant Cell Reports Springer-Verlag 25/5(2006-05-01), 410-416 0721-7714 25:5<410 2006 25 299 https://doi.org/10.1007/s00299-005-0067-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0067-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Aionesei Tatiana Max F. Perutz Laboratories at the Vienna Biocenter, Department of Genetics, Vienna University, Dr. Bohrgasse 9/4, A-1030, Vienna, Austria aut NATIONALLICENCE 700 1- Hosp Julia Max F. Perutz Laboratories at the Vienna Biocenter, Department of Genetics, Vienna University, Dr. Bohrgasse 9/4, A-1030, Vienna, Austria aut NATIONALLICENCE 700 1- Voronin Viktor Max F. Perutz Laboratories at the Vienna Biocenter, Department of Genetics, Vienna University, Dr. Bohrgasse 9/4, A-1030, Vienna, Austria aut NATIONALLICENCE 700 1- Heberle-Bors Erwin Max F. Perutz Laboratories at the Vienna Biocenter, Department of Genetics, Vienna University, Dr. Bohrgasse 9/4, A-1030, Vienna, Austria aut NATIONALLICENCE 700 1- Touraev Alisher Max F. Perutz Laboratories at the Vienna Biocenter, Department of Genetics, Vienna University, Dr. Bohrgasse 9/4, A-1030, Vienna, Austria aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/5(2006-05-01), 410-416 0721-7714 25:5<410 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer