caa a22 4500 467901236 CHVBK 20180406152818.0 cr unu---uuuuu 170328e20060501xx s 000 0 eng 10.1007/s00299-005-0051-z doi (NATIONALLICENCE)springer-10.1007/s00299-005-0051-z The maize Knotted1 gene is an effective positive selectable marker gene for Agrobacterium -mediated tobacco transformation [Elektronische Daten] [Keming Luo, Xuelian Zheng, Yongqin Chen, Yuehua Xiao, Degang Zhao, Richard McAvoy, Yan Pei, Yi Li] We have assessed the use of a homeobox gene knotted1 (kn1) from maize as a selectable marker gene for plant transformation. The kn1 gene under the control of cauliflower mosaic virus 35S promoter (35S::kn1) was introduced into Nicotiana tabacum cv. Xanthi via Agrobacterium-mediated transformation. Under nonselective conditions (without antibiotic selection) on a hormone-free medium (MS), a large number of transgenic calli and shoots were obtained from explants that were infected with Agrobacterium tumefaciens LBA4404 harboring the 35S::kn1 gene. On the other hand, no calli or shoots were produced from explants that were infected with an Agrobacterium strain harboring pBI121 (nptII selection) or from uninfected controls cultured under identical conditions. Relative to kanamycin selection conferred by nptII, the use of kn1 resulted in a 3-fold increase in transformation efficiency. The transgenic status of shoots obtained was confirmed by both histochemical detection of GUS activity and molecular analysis. The results presented here suggest that kn1 gene could be used as an effective alternative selection marker with a potential to enhance plant transformation efficiency in many plant species. With kn1 gene as a selection marker gene, no antibiotic-resistance or herbicide-resistance genes are needed so that potential risks associated with the use of these traditional selection marker genes can be eliminated. Springer-Verlag, 2005 Transformation nationallicence Selectable marker nationallicence kn1 gene nationallicence ipt gene nationallicence CaMV 35S : cauliflower mosaic virus 35S nationallicence kn1 : Knotted1 gene nationallicence ipt : Isopentenyl transferase nationallicence BA : Benzylaminopurine nationallicence NAA : Naphthaleneacetic acid nationallicence NPTII : Neomycin phosphotransferase nationallicence MS Medium : Musashige & Skoog medium nationallicence GUS : β-glucuronidase nationallicence Luo Keming Biotechnology Research Center, Southwest University, 400716, Chongqing, P.R. China aut Zheng Xuelian Biotechnology Research Center, Southwest University, 400716, Chongqing, P.R. China aut Chen Yongqin Department of Biotechnology, College of Life Sciences, Hubei University, 430062, Wuhan, P.R. China aut Xiao Yuehua Biotechnology Research Center, Southwest University, 400716, Chongqing, P.R. China aut Zhao Degang Key Laboratory of Agricultural Biotechnology, Guizhou University, 550025, Guizhou, P.R. China aut McAvoy Richard Department of Plant Science, University of Connecticut, 06269, Storrs, CT, USA aut Pei Yan Biotechnology Research Center, Southwest University, 400716, Chongqing, P.R. China aut Li Yi Department of Plant Science, University of Connecticut, 06269, Storrs, CT, USA aut Plant Cell Reports Springer-Verlag 25/5(2006-05-01), 403-409 0721-7714 25:5<403 2006 25 299 https://doi.org/10.1007/s00299-005-0051-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0051-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Luo Keming Biotechnology Research Center, Southwest University, 400716, Chongqing, P.R. China aut NATIONALLICENCE 700 1- Zheng Xuelian Biotechnology Research Center, Southwest University, 400716, Chongqing, P.R. China aut NATIONALLICENCE 700 1- Chen Yongqin Department of Biotechnology, College of Life Sciences, Hubei University, 430062, Wuhan, P.R. China aut NATIONALLICENCE 700 1- Xiao Yuehua Biotechnology Research Center, Southwest University, 400716, Chongqing, P.R. China aut NATIONALLICENCE 700 1- Zhao Degang Key Laboratory of Agricultural Biotechnology, Guizhou University, 550025, Guizhou, P.R. China aut NATIONALLICENCE 700 1- McAvoy Richard Department of Plant Science, University of Connecticut, 06269, Storrs, CT, USA aut NATIONALLICENCE 700 1- Pei Yan Biotechnology Research Center, Southwest University, 400716, Chongqing, P.R. China aut NATIONALLICENCE 700 1- Li Yi Department of Plant Science, University of Connecticut, 06269, Storrs, CT, USA aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/5(2006-05-01), 403-409 0721-7714 25:5<403 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer