caa a22 4500 467901244 CHVBK 20180406152818.0 cr unu---uuuuu 170328e20060501xx s 000 0 eng 10.1007/s00299-005-0098-x doi (NATIONALLICENCE)springer-10.1007/s00299-005-0098-x Oligonucleotide-directed gene repair in wheat using a transient plasmid gene repair assay system [Elektronische Daten] [Chongmei Dong, Peter Beetham, Kate Vincent, Peter Sharp] Oligonucleotide-directed gene repair is a potential technique for agricultural trait modification in economically important crops. However, large variation in the repair frequencies among the scientific reports indicates that there are many factors influencing the repair process. We report here a transient assay system using GFP as a reporter for testing the efficiency of plasmid DNA repair in cultured wheat cells. This assay showed that osmotic medium supplemented with 2,4-D increased the oligo-targeting frequency, and that the repair of a point mutation was more efficient than repair of a single base deletion mutation in cultured scutellum cells of immature wheat embryos. This study provides the first evidence that oligonucleotide-directed mutagenesis is applicable to regenerable cultured wheat scutellum cells. Springer-Verlag, 2005 Gene repair nationallicence GFP nationallicence Immature embryo nationallicence Plasmid DNA nationallicence Single-stranded DNA oligonucleotide nationallicence Wheat nationallicence Dong Chongmei Plant Breeding Institute, University of Sydney, PMB 11, 2570, Camden, NSW, Australia aut Beetham Peter Cibus Genetics LLC, 4025 Sorrento Valley Blvd., 92121, San Diego, CA, USA aut Vincent Kate Plant Breeding Institute, University of Sydney, PMB 11, 2570, Camden, NSW, Australia aut Sharp Peter Plant Breeding Institute, University of Sydney, PMB 11, 2570, Camden, NSW, Australia aut Plant Cell Reports Springer-Verlag 25/5(2006-05-01), 457-465 0721-7714 25:5<457 2006 25 299 https://doi.org/10.1007/s00299-005-0098-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0098-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dong Chongmei Plant Breeding Institute, University of Sydney, PMB 11, 2570, Camden, NSW, Australia aut NATIONALLICENCE 700 1- Beetham Peter Cibus Genetics LLC, 4025 Sorrento Valley Blvd., 92121, San Diego, CA, USA aut NATIONALLICENCE 700 1- Vincent Kate Plant Breeding Institute, University of Sydney, PMB 11, 2570, Camden, NSW, Australia aut NATIONALLICENCE 700 1- Sharp Peter Plant Breeding Institute, University of Sydney, PMB 11, 2570, Camden, NSW, Australia aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/5(2006-05-01), 457-465 0721-7714 25:5<457 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer