caa a22 4500 467901252 CHVBK 20180406152818.0 cr unu---uuuuu 170328e20060501xx s 000 0 eng 10.1007/s00299-005-0100-7 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0100-7 A tissue culture system for different germplasms of indica rice [Elektronische Daten] [Xiaojia Ge, Zhaohui Chu, Yongjun Lin, Shiping Wang] Agrobacterium-mediated transformation of indica rice has been manipulated in only a limited number of cultivars because the majority of indica varieties are recalcitrant to in vitro response. Establishment of a highly efficient and widely used tissue culture system for indica rice will accelerate the application of transformation technology in breeding programs and the study of the functions of indica-specific genes. By manipulating plant growth regulators, organic components and salts within the culture media, we established two media for callus induction and subculture, respectively, in tissue culture of indica rice. The modified media could guarantee the production and proliferation of a great number of embryogenic calli with high regeneration capacity from mature seeds representing different indica rice germplasms. The calli obtained from this system should be ideal material for Agrobacterium-mediated transformation. The results suggest that this optimized tissue culture system will be widely applicable for the tissue culture of indica varieties. Springer-Verlag, 2005 Callus nationallicence Induction nationallicence Subculture nationallicence Agrobacterium -mediated transformation nationallicence Indica rice nationallicence Ge Xiaojia National Key Laboratory of Crop Genetic Improvement, National Center for Gene Research (Wuhan), Huazhong Agricultural University, 430070, Wuhan, P. R. China aut Chu Zhaohui National Key Laboratory of Crop Genetic Improvement, National Center for Gene Research (Wuhan), Huazhong Agricultural University, 430070, Wuhan, P. R. China aut Lin Yongjun National Key Laboratory of Crop Genetic Improvement, National Center for Gene Research (Wuhan), Huazhong Agricultural University, 430070, Wuhan, P. R. China aut Wang Shiping National Key Laboratory of Crop Genetic Improvement, National Center for Gene Research (Wuhan), Huazhong Agricultural University, 430070, Wuhan, P. R. China aut Plant Cell Reports Springer-Verlag 25/5(2006-05-01), 392-402 0721-7714 25:5<392 2006 25 299 https://doi.org/10.1007/s00299-005-0100-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0100-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ge Xiaojia National Key Laboratory of Crop Genetic Improvement, National Center for Gene Research (Wuhan), Huazhong Agricultural University, 430070, Wuhan, P. R. China aut NATIONALLICENCE 700 1- Chu Zhaohui National Key Laboratory of Crop Genetic Improvement, National Center for Gene Research (Wuhan), Huazhong Agricultural University, 430070, Wuhan, P. R. China aut NATIONALLICENCE 700 1- Lin Yongjun National Key Laboratory of Crop Genetic Improvement, National Center for Gene Research (Wuhan), Huazhong Agricultural University, 430070, Wuhan, P. R. China aut NATIONALLICENCE 700 1- Wang Shiping National Key Laboratory of Crop Genetic Improvement, National Center for Gene Research (Wuhan), Huazhong Agricultural University, 430070, Wuhan, P. R. China aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/5(2006-05-01), 392-402 0721-7714 25:5<392 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer